Histochemical Techniques
About this book
Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.
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Information
Table of contents
- Front Cover
- Histochemical Techniques
- Copyright Page
- Table of Contents
- FOREWORD
- PREFACE
- Chapter 1. Introduction
- Chapter 2. Fixation
- Chapter 3. Paraffin Sections
- Chapter 4. Frozen Sections
- Chapter 5. Cryostats
- Chapter 6. Freeze Drying and Freeze Substitution
- Chapter 7. Carbohydrates
- Chapter 8. Proteins and Amino Acids
- Chapter 9. Amyloid
- Chapter 10. Lipids
- Chapter 11. Nucleic Acids
- Chapter 12. Pigments
- Chapter 13. Minerals
- Chapter 14. Principles of Enzyme Histochemistry
- Chapter 15. Phosphatases
- Chapter 16. Esterases
- Chapter 17. Oxidoreductases: I. Oxidases
- Chapter 18. Oxidoreductases: II. Dehydrogenases
- Chapter 19. Miscellaneous Techniques
- Chapter 20. Ultra-histochemis try
- APPENDIX
- Index
