Colloidal Gold
eBook - PDF

Colloidal Gold

Principles, Methods, and Applications

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  1. 421 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Colloidal Gold

Principles, Methods, and Applications

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About this book

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. - Among the first books to cover principles and methodology of colloidal gold in microscopy - Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book - Discusses problems and limitations of techniques - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields - Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins - Preserving and localizing antigenicity; sectioning; high-resolution morphological localization - Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation - Applications to biomedical studies - Colloidal gold in conjunction with video-enhanced microscopy

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Table of contents

  1. Front Cover
  2. Colloidal Gold: Principles, Methods, and Applications
  3. Copyright Page
  4. Table of Contents
  5. Contributors
  6. Preface
  7. Contents of Other Volumes
  8. Chapter 1. Some Basic Concepts for the Choice of Methods
  9. Chapter 2. Techniques for the Production of Monoclonal and Polyclonal Antibodies
  10. Chapter 3. Lowicryl Resins
  11. Chapter 4. Polar or Apolar Lowicryl Resin for Immunolabeling
  12. Chapter 5. Yield of Immunolabel Compared to Resin Sections and Thawed Cryosections
  13. Chapter 6. Quantitation of Colloidal Gold b y Electron Microscopy
  14. Chapter 7. Colloidal Gold Labeling for Determining Cell Surface Area
  15. Chapter 8. Simultaneous Demonstration of Antigens on Outer and Protoplasmic Surfaces of the Plasma Membrane by Replica Immunocytochemistry
  16. Chapter 9. Double Labeling of Antigenic Sites on Cell Surfaces Imaged with Backscattered Electrons
  17. Chapter 10. Location and Identification of Colloidal Gold Particles with an Energy Dispersive Analyzer
  18. Chapter 11. Use of Dinitrophenol IgG Conjugates: Immunogold Labeling of Cellular Antigens on Thin Sections of Osmicated and Epon- Embedded Specimens
  19. Chapter 12. Preparation and Application of Insulin – Gold Complex
  20. Chapter 13. Microinjection of Colloidal Gold
  21. Chapter 14. Combined Diaminobenzidine—Colloidal Gold Staining
  22. Chapter 15. Colloidal Gold Techniques and the Immunocytochemistry of Antigen–Antibody Reactions at Interfaces
  23. Chapter 16. Colloidal Gold Labeling for Diagnostic Pathology
  24. Chapter 17. Immunogold - Silver Staining Using Microwave Irradiation
  25. Chapter 18. A Correlative Approach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low Voltage Scanning Electron Microscopy, and High-Voltage Electron Microscopy
  26. Index