Recombinant DNA Laboratory Manual, Revised Edition
eBook - PDF

Recombinant DNA Laboratory Manual, Revised Edition

  1. 224 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Recombinant DNA Laboratory Manual, Revised Edition

About this book

The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. - Comb-bound, three-column, large 9-1/4" x 7-1/2" format - Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts - Necessary reagents and equipment are presented in a checklist at the beginning of each protocol - Techniques for bacteria are complemented with those for Drosophila - Each experiment has been tested in the laboratory by students for five years - Features a complete chapter on computers in the molecular biology laboratory - Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains

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Yes, you can access Recombinant DNA Laboratory Manual, Revised Edition by Judith W. Zyskind,Sanford I. Bernstein in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Microbiology. We have over one million books available in our catalogue for you to explore.

Table of contents

  1. Front Cover
  2. Recombinant DNA Laboratory Manual
  3. Copyright Page
  4. Table of Contents
  5. Dedication
  6. PREFACE
  7. SCHEDULE OF LABORATORY EXERCISES
  8. Lab I. BACTERIAL GROWTH PARAMETERS
  9. Lab II. Isolation and Analysis of Bacterial and Drosophila Chromosomal DNA
  10. Lab III. Plasmid DNA Isolation and Agarose Gel Analysis
  11. Lab IV. Introduction of DNA into Cells
  12. Lab V. Tn5 Mutagenesis of PBR329
  13. Lab VI. DNA Cloning in M13
  14. Lab VII. DNA Sequencing
  15. Lab VIII. DNA Gel Blotting, Probe Preparation, Hybridization, and Hybrid Detection
  16. Lab IX. Lambda Phage Manipulations
  17. Lab X. The Polymerase Chain Reaction Technique
  18. Appendix 1: USE OF COMPUTERS IN A MOLECULAR BIOLOGY LABORATORY
  19. Appendix 2: SAFETY IN THE RECOMBINANT DNA LABORATORY
  20. Appendix 3: SOLUTIONS
  21. Appendix 4: STRAINS AND DNA
  22. Appendix 5: MOLECULAR BIOLOGY REAGENT SUPPLIERS
  23. Appendix 6: FREQUENTLY USED ENZYMES
  24. Appendix 7: FREQUENTLY USED TECHNIQUES NOT DESCRIBED IN LABS
  25. INDEX