Male Reproductive Toxicology

Robert E. Chapin, Jerrold J. Heindel, Robert E. Chapin, Jerrold J. Heindel

• Publisher: Academic Press
• Year: 2013
• Print ISBN:
  9780124612075
• eBook ISBN:
  9781483270951
• Topic: Medicine
• Subtopic: Pharmacology

Methods in Toxicology, Volume 3: Male Reproductive Toxicology, Part A, deals with the male reproductive system and discusses methods that will help identify toxicant-induced changes at all levels in living organisms. It is important to realize that a toxic effect does not occur in a vacuum. All work in toxicology must be predicated on a demonstrated adverse effect in vivo. If good toxicology cannot exist in a vacuum, then there must be a structure. Thus, the book begins by presenting a few models as examples of the ways experiments could be grouped to define the toxicity of a chemical. This is followed by separate chapters on methods such as male mouse sexual behavior test; in vitro techniques for assessing pituitary secretory function; histological methods for preservation of the rat testis; procedures for assessing testicular sperm head counts in mice, rats, and dogs; and guidelines for conducting rodent dominant lethal tests. Subsequent chapters cover topics such as methods for the isolation and purification of Leydig cells from rat and mouse testes, and techniques used in semen analysis and fertility assessment in the rabbit.

Table of contents

• Front Cover
• Male Reproductive Toxicology, Part A
• Copyright Page
• Table of Contents
• Contributors
• Chapter 1. Introduction
• Chapter 2. Male Mouse Sexual Behavior Test
• Chapter 3. In Vitro Techniques for Assessing Pituitary Secretory Function
• Chapter 4. Histological Methods for Evaluation of the Testis
• Chapter 5. Application of Testicular Sperm Head Counts in the Assessment of Male Reproductive Toxicity
• Chapter 6. Stage Synchronization in Rat Seminiferous Tubules Using Vitamin A Depletion and Repletion
• Chapter 7. Spermatogonial Stem Cells: Assessing Their Survival and Ability to Produce Differentiated Cells
• Chapter 8. Dominant Lethal Tests in Male and Female Mice
• Chapter 9. Transillumination–Phase-Contrast Microscopic Techniques for Evaluation of Male Germ Cell Toxicity and Mutagenicity
• Chapter 10. Isolation and Culture of Leydig Cells from Adult Rats
• Chapter 11. Isolation and Primary Culture of Leydig Cells
• Chapter 12. Purification and Primary Culture of Leydig Cells
• Chapter 13. Preparation and Use of Sertoli Cell-Enriched Cultures from 18-Day-Old Rats
• Chapter 14. Two-Compartment Cultures of Sertoli Cells—Applications in Testicular Toxicology
• Chapter 15. Isolation, Separation, and Short-Term Culture of Spermatogenic Cells
• Chapter 16. Collection of Interstitial Fluid and Seminiferous Tubule Fluid from the Rat Testis
• Chapter 17. Isolation and Culture of Epididymal Epithelial Cells from Adult Rats
• Chapter 18. Evaluation of Protein Synthesis by the Epididymis
• Chapter 19. Utilizing Cryo Resources CellSoft Computer-Assisted Sperm Analysis System for Rat Sperm Motility Studies
• Chapter 20. Computer-Assisted Sperm Analysis of Rodent Epididymal Sperm Motility Using the Hamilton-Thorn Motility Analyzer
• Chapter 21. Methods for Evaluation of Rat Epididymal Sperm Morphology
• Chapter 22. Semen Analysis and Fertility Assessment in the Rabbit
• Chapter 23. General Techniques for Assessing Male Reproductive Potential in Human Field Studies
• Index