Handbook of HLA Typing Techniques
eBook - ePub

Handbook of HLA Typing Techniques

Kam M. Hui, Jeffrey L. Bidwell

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  1. 420 pages
  2. English
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eBook - ePub

Handbook of HLA Typing Techniques

Kam M. Hui, Jeffrey L. Bidwell

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About This Book

This book presents an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. It includes detailed methodologies, notes on the interpretation of tests, reference material, and appendices.

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Information

Publisher
CRC Press
Year
2020
ISBN
9781000153392

1

PCR-RFLP

INTRODUCTION

The authors have developed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method based on the digestion of PCR-amplified DNAs with allele-specific enzymes as a reliable, convenient, and practical HLA class II DNA typing technique,1, 2, 3, 4, 5, 6, 7 rather than being based on hybridization with multiple sequence-specific oligonucleotide probes (PCR-SSO) (Figure 1). The modified PCR-RFLP method incorporating informative enzymes, which have a single recognition site in some alleles but none in other alleles in the amplified regions, is simpler and more sensitive because genotypes...

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