Handbook of HLA Typing Techniques

Kam M. Hui, Jeffrey L. Bidwell

• Publisher: CRC Press
• Year: 2020
• eBook ISBN:
  9781000153392
• Topic: Medicine
• Subtopic: Genetics in Medicine

This book presents an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. It includes detailed methodologies, notes on the interpretation of tests, reference material, and appendices.

1

PCR-RFLP

INTRODUCTION

The authors have developed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method based on the digestion of PCR-amplified DNAs with allele-specific enzymes as a reliable, convenient, and practical HLA class II DNA typing technique,^{1, 2, 3, 4, 5, 6, 7} rather than being based on hybridization with multiple sequence-specific oligonucleotide probes (PCR-SSO) (Figure 1). The modified PCR-RFLP method incorporating informative enzymes, which have a single recognition site in some alleles but none in other alleles in the amplified regions, is simpler and more sensitive because genotypes...

Table of contents

• Cover
• Title Page
• Copyright Page
• The Editors
• Contributors
• Table of Contents
• Introduction HLA Matching in Transplantation
• Chapter 1 PCR-RFLP
• Chapter 2 HLA-DR and -DQ Typing by DNA-RFLP Analysis
• Chapter 3 Rapid HLA-DR-Dw and DP Matching by PCR Fingerprinting and Related DNA Heteroduplex Technologies
• Chapter 4 A Comprehensive HLA-DRB, -DQB, and -DPB Oligotyping Procedure by Hybridization with Sequence-Specific Oligonucleotide Probes
• Chapter 5 HLA-DR Typing by Polymerase Chain Reaction Amplification with Sequence-Specific Primers (PCR-SSP)
• Chapter 6 Serological Typing of HLA-A, -B, and -C Antigens
• Chapter 7 HLA-DR and -DQ Serotyping
• Chapter 8 Definition of HLA-Dw Determinants Using Homozygous Typing Cells and the Mixed Lymphocyte Culture
• Chapter 9 Definition of HLA-Dw and HLA-DPw Determinants by the Primed Lymphocyte Test
• Appendix HLA Class II Nucleotide Sequences, 1992
• Index