
- 184 pages
- English
- PDF
- Available on iOS & Android
Polymerase Chain Reaction for Biomedical Applications
About this book
Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.
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Table of contents
- Polymerase Chain Reaction for Biomedical Applications
- Contents
- Preface
- Chapter 1 Guidelines for Successful Quantitative Gene Expression in Real- Time qPCR Assays
- Chapter 2 High-Throughput Platforms in Real-Time PCR and Applications
- Chapter 3 Hot Cell-Direct PCR Aimed at Specific Cell Detection
- Chapter 4 Regulatory Concern of Polymerase Chain Reaction (PCR) Carryover Contamination
- Chapter 5 Multiplex Polymerase Chain Reaction Assay for Early Diagnosis of Viral Infection
- Chapter 6 The Advantages of Using Multiplex PCR for the Simultaneous Detection of Six Sexually Transmitted Diseases
- Chapter 7 - A Real-Time PCR-Based Diagnostic Test for Organismsin Respiratory Tract Infection
- Chapter 8 - PCR Technique for the Microbial Analysis of Inanimate Hospital Environment
- Chapter 9 - PCR: A Powerful Method in Food Safety Field
- Chapter 10 - Site‐Directed Mutagenesis by Polymerase Chain Reaction