Antibody Engineering
eBook - PDF

Antibody Engineering

  1. 312 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Antibody Engineering

About this book

Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.

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Yes, you can access Antibody Engineering by Thomas Böldicke in PDF and/or ePUB format, as well as other popular books in Medicine & Genetics in Medicine. We have over one million books available in our catalogue for you to explore.

Information

Publisher
IntechOpen
Year
2018
Print ISBN
9789535138259
eBook ISBN
9789535139751
Topic
Medicine
Subtopic
Genetics in Medicine

Table of contents

  1. Antibody Engineering
  2. Contents
  3. Preface
  4. Chapter 1 Generation of Antibody Diversity
  5. Chapter 2 High Affinity Maturated Human Antibodies from Naïve and Synthetic Antibody Repertoires
  6. Chapter 3 Display Technologies for the Selection of Monoclonal Antibodies for Clinical Use
  7. Chapter 4 Detailed Protocols for the Selection of Antiviral Human Antibodies from Combinatorial Immune Phage Display Libraries
  8. Chapter 5 Colony Assay for Antibody Library Screening: Outlook and Comparison to Display Screening
  9. Chapter 6 Construction and Characteristics of a Recombinant Single- Chain Antibody Fragment against Bacterial Type III Secretion
  10. Chapter 7 Separation of Monoclonal Antibodies by Analytical Size Exclusion Chromatography
  11. Chapter 8 The Development of Single Domain Antibodies for Diagnostic and Therapeutic Applications
  12. Chapter 9 Use, Applications and Mechanisms of Intracellular Actions of Camelid VHHs
  13. Chapter 10 Structural Diversity Problems and the Solving Method for Antibody Light Chains
  14. Chapter 11 Immune-Mediated Skin Reactions Induced by Recombinant Antibodies and Other TNF-Alpha Inhibitors
  15. Chapter 12 Bioinformatics as a Tool to Identify Infectious Disease Pathogen Peptide Sequences as Targets for Antibody Engineering