Forensic Entomology

The Utility of Arthropods in Legal Investigations, Third Edition

Jason H. Byrd, Jeffery K. Tomberlin, Jason H. Byrd, Jeffery K. Tomberlin

• Publisher: CRC Press
• Year: 2019
• eBook ISBN: 9781351163743
• Edition: 3
• Topic: Social Sciences
• Subtopic: Forensic Science
• Index: Social Sciences

Forensic Entomology: The Utility of Arthropods in Legal Investigations, Third Edition continues in the tradition of the two best-selling prior editions and maintains its status as the single-most comprehensive book on Forensic Entomology currently available. It includes current, in-the-field best practices contributed by top professionals in the field who have advanced it through research and fieldwork over the last several decades.

The use of entomology in crime scene and forensic investigations has never been more prevalent or useful given the work that can be done with entomological evidence. The book recounts briefly the many documented historical applications of forensic entomology over several thousand years. Chapters examine the biological foundations of insect biology and scientific underpinnings of forensic entomology, the principles that govern utilizing insects in legal and criminal investigations. The field today is diverse, both in topics studied, researched and practiced, as is the field of professionals that has expanded throughout the world to become a vital forensic sub-discipline.

Forensic Entomology, Third Edition celebrates this diversity by including several new chapters by premier experts in the field that covers such emerging topics as wildlife forensic entomology, microbiomes, urban forensic entomology, and larval insect identification, many of which are covered in depth for the first time. The book will be an invaluable reference for investigators, legal professionals, researchers, practicing and aspiring forensic entomologists, and for the many students enrolled in forensic science and entomology university programs.

CHAPTER 1

GENERAL ENTOMOLOGY AND BASIC ARTHROPOD BIOLOGY

Adrienne Brundage

Introduction

Arthropod and Insect Classification

Arthropoda

Basic Insect Anatomy

Overall Body Form

The Exoskeleton

Head

Eyes

Antennae

Major Antennal Types Found in Insects

Mouthparts

Thorax

Legs and Wings

Abdomen

Internal Anatomy

The Insect Life Cycle

Types of Reproduction

Types of Metamorphosis

Ametabolous Metamorphosis

Paurometabolous Metamorphosis

Hemimetabolous Metamorphosis

Holometabolous Metamorphosis

Temperature

Conclusions

References

INTRODUCTION

Entomology is the study of those six-legged, ubiquitous organisms called insects and often includes closely related arthropods, such as mites, ticks, and spiders. This is a huge field of study and involves an entire range of biological disciplines ranging from taxonomy and physiology to genetics and evolution; the common denominator among all these different areas is that arthropods are the focus of study.

Arthropods have a variety of benefits that are both tangible and intangible. They are the most diverse and abundant group of organisms on earth. There are approximately 1.8 million described species of animals on earth, with an estimated 8.7 million species total. (Mora et al. 2011). The vast majority of these described animals, approximately one million, are insects (Stork 2018) (Figure 1.1).

Arthropods are found in just about every habitat on the planet, oftentimes partitioning that habitat into niches that they share with other species, which ultimately leads to great diversity through natural selection. Of the estimated 8.7 million described and undescribed animal species on earth, the vast majority (approximately 73%) are thought to be insects (Mora et al. 2011; Stork 2018). This diversity leads to the near universal presence in every habitat type, doing every imaginal job. Insects are the basic components of the food web, acting as primary and secondary consumers, predators, parasites, and decomposers. Insects act as pollinators—they transfer pollen from one flower to another, allowing plants to set seed and fruit. Insects produce tangible products, used by humans every day. We can thank insects for everything from honey and bees wax to dyes and pharmaceuticals. There is even a push to use insects as a food source in and of themselves.

Such extreme diversity is achieved by several factors. First, their size has allowed them to exploit a vast number of habitats not available to larger animals. Most insects are small (1 cm or less), so they have a finer grained environment. This means they can exploit very tiny habitats resulting in insects that specialize in consuming roots and leaves on flower buds (Figure 1.2a and b).

Figure 1.1 Insects account for the majority of described species on earth, accounting for more species than all other organisms combined.

Arthropods also have a highly organized, complex neuro-motor system. In some instances, their neuro-physiological capabilities provide greater ecological plasticity enabling them to survive in changing environments. Wings are limited largely to insects and greatly facilitate dispersal which is a great advantage when resources are depleted. This ability ultimately allows for mating success and specialization.

Rapid development cycles and short generations allow for rapid evolution and quick response to changing environments and the ability to quickly exploit new niches. Metamorphosis reduces competition for resources between adults and immatures as they rely on different resources for survival. Long-term rapid development and metamorphosis during interactions with other organisms leads to coevolution. Insects and other arthropods become more diverse via bottom-up (plant defenses), top down (predators, parasitoids, and pathogens), and horizontal (competition) forces. Overall, insects have touched every aspect of our lives throughout our evolutionary history (Scudder 2009).

Figure 1.2 (a) Fairyflies (Hymenoptera: Chalcidoidea) are some of the smallest flying insects, ranging from 0.139 to 1 mm in length. (b) The titan beetle (Coleoptera: Cerambycidae) is one of the largest insects, ranging from 16 to 17 cm. ([a] Huber, J.T. and Huber, J.T., ZooKeys, 345, 47–72, 2013; [b] Didier Descouens—Own work, https://commons.wikimedia.org/w/index.php?curid=16917907.)

ARTHROPOD AND INSECT CLASSIFICATION

Arthropoda

All arthropods have several basic characteristics in common: bilateral symmetry; segmented external appendages; an exoskeleton formed from chitin, or long-chain polymer of N-acetylglucosamine, a derivative of glucose; and external segmentation (Figure 1.3). Arthropods are broken up into several groups based on their external characteristics. Insecta is a class of Arthropoda. One can differentiate insects from all other arthropods based on the presence of three major body regions (head, thorax, and abdomen); a single pair of antennae, often modified for specialized functions; and three pairs of segmented legs (Gullan and Cranston 2009).

Several other Arthropoda classes are also studied by entomologists. These taxa include the Arachnida (spiders, scorpions, ticks, and mites), the Diplopoda (millipedes), and the Chilopoda (centipedes). Most of these groups when associated with a crime scene are incidental. Spiders, scorpions, and centipedes may be predating upon other organisms present. Millipedes and many types of mites are plant feeders, which use the scene as a general habitat. Ticks and parasitic mites may be associated with living individuals, but only briefly after death; these groups are obligate parasites and require a living organism to survive. After death, they will vacate the body very quickly. By far the most common and currently most useful group to be associated with crime scenes are predominantly within the Insecta.

Figure 1.3 Chemical structure of N-acetylglucosamine (C₈H₁₅NO₆). (Royal Society of Chemistry, http://www.rsc.org/help-legal/legal/copyright-permissions/.)

Basic Insect Anatomy

Insect anatomy is the foundation of entomology. As previously mentioned, arthropods in general have evolved strategies to live successfully under a variety of conditions, and a strong understanding of external and internal anatomy of the insects involved in decomposition gives the investigator a good basis to understand insect behavior.

Overall Body Form

The overall body form of an insect is cylindrical and elongate and is broken up into three major body regions: the head, the thorax, and the abdomen. This is the overall body form of an insect, as illustrated by a grasshopper (Orthoptera: Acrididae) (Figure 1.4).

Grasshopper bodies are ancient and are therefore representative of ancestral insect forms. This makes the grasshopper an excellent comparative model.

The overall body form of the basal insect is cylindrical and elongate. It has a robust appearance, with an internal body cavity, the haemocoel, formed by the exoskeleton and filled with hemolymph or insect blood. Externally, the plan is straightforward: there is an anterior head, legs and wings in the center, and an abdomen and reproductive organs posteriorly (Figure 1.5). The bodily structure is maintained through the exoskeleton.

Figure 1.4 Typical insect body morphology showing the head, thorax,and abdomen. Eastern lubber grasshopper, Romalea microptera (Orthoptera: Acrididae). (http://citeseerx.ist.psu.edu/viewdoc/download? doi=10.1.1.518.6402&rep=rep1&type=pdf.)

Figure 1.5 Diagram of the major body regions of a winged insect. (From Manual of Neartic Diptera, vol. 1.)

THE EXOSKELETON

An exoskeleton is the external surface of the insect. This exoskeleton is stiff, and the form of the exoskeleton is primarily due to its physical make up. It consists of chitin, which is a long-chain polymer of N-acetylglucosamine a derivative of the sugar glucose. In its unmodified form, chitin is translucent, pliable, resilient, and durable. However, it is heavily modified in arthropods. It is embedded with sclerotin, a tanned protein matrix, which lends a stiffness and a hardness to the exoskeleton. This is why the shell of a crab is difficult to crack or why a cockroach (Blattodea: Blattidae) crunches when you step on it. The amount of sclerotin in the exoskeleton depends on the species, which means different arthropods can have different pliability in their exoskeletons (Campbell 1929).

The chemical makeup of the exoskel...

Table of contents

• Cover
• Half Title
• Title Page
• Copyright Page
• Table of Contents
• In Memoriam
• Foreword
• Preface
• Acknowledgments
• Prologue
• Editors
• Contributors
• Introduction: Current Perceptions and Status of Forensic Entomology
• Chapter 1 General Entomology and Basic Arthropod Biology
• Chapter 2 Insects of Forensic Importance
• Chapter 3 Entomological Evidence Collections Methods: American Board of Forensic Entomology Approved Protocols
• Chapter 4 Laboratory-Rearing of Forensic Insects
• Chapter 5 Factors That Influence Insect Succession on Carrion
• Chapter 6 Invertebrate Succession in Natural Terrestrial Environments
• Chapter 7 The Role of Aquatic Organisms in Forensic Investigations
• Chapter 8 Recovering Buried Bodies and Surface Scatter: The Associated Anthropological, Botanical, and Entomological Evidence
• Chapter 9 Estimating the Postmortem Interval
• Chapter 10 Insect Development as It Relates to Forensic Entomology
• Chapter 11 Molecular Genetic Methods for Forensic Entomology
• Chapter 12 The Soil Environment and Forensic Entomology
• Chapter 13 Advances in Entomotoxicology: Weaknesses and Strengths
• Chapter 14 Is PMI the Hypothesis or the Null Hypothesis?
• Chapter 15 The Forensic Entomologist as Expert Witness
• Chapter 16 Livestock Entomology
• Chapter 17 Ecological Theory of Community Assembly and Its Application in Forensic Entomology
• Chapter 18 Forensic Meteorology: The Science of Applying Weather Observations to Civil and Criminal Litigation
• Chapter 19 Entomological Alteration of Bloodstain Evidence
• Chapter 20 Keys to the Genera and Species of Blow Flies (Diptera: Calliphoridae) of America, North of Mexico
• Chapter 21 The Use of Entomological Evidence in Analyzing Cases of Neglect and Abuse in Humans and Animals
• Chapter 22 Acarology in Crimino-Legal Investigations: The Human Acarofauna During Life and Death
• Chapter 23 Wildlife Forensic Entomology
• Chapter 24 The Role of Decomposition Volatile Organic Compounds in Chemical Ecology
• Chapter 25 Forensic Entomology and the Microbiome
• Chapter 26 Urban Entomology
• Chapter 27 Larvae of the North American Calyptratae Flies of Forensic Importance
• Chapter 28 The Professional History of Forensic Entomology
• Chapter 29 Practical Considerations for Teaching Forensic Entomology
• Index