This Spotlight reviews non-invasive optical methods to determine the blood flow velocity in microcirculation, with an emphasis on the most recent developments, that may be routinely applied in cellular biology laboratories. The methods covered in this book include both scanning and whole-field optical imaging techniques based on endogenous or exogenous (fluorescent) contrast agents for plasma and red-blood-cell visualization, such as laser Doppler velocimetry, laser speckle contrast imaging, diffuse correlation spectroscopy, particle image velocimetry, and two-photon microscopy. Several methods have been developed to quantify the blood flow velocity within microvessels by exploiting the cross-correlation concept applied to optical scanning microscopes: dual-foci cross-correlation, spatio-temporal image correlation spectroscopy, line-scanning techniques, and a recently developed single raster-scanned xy-image technique (FLICS). The basic operational principles, the advantages and disadvantages, and the technical issues of these methods will be discussed and compared, and their applications in biomedical research will be presented.
- 100 pages
- English
- PDF
- Available on iOS & Android
eBook - PDF
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Information
Topic
MedicineSubtopic
Diagnostics ImagingTable of contents
- Copyright
- Series Page
- Preface
- 1 Introduction
- 2 Laser Speckle Contrast Imaging
- 3 Doppler Optical Coherence Tomography
- 4 Diffuse Correlation Spectroscopy
- 5 Multiplexed and Image Correlation Methods: Video-Based Acquisition
- 6 Space–Time Diagrams and Image Correlation Methods: Scanning-Based Acquisition and Detection
- References
- Author Biographies
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Yes, you can access Optical Blood Flow Measurement in Microcirculatory Systems by Sironi, Laura,D'Alfonso, Laura,Bouzin, Margaux,Ceffa, Nicolo' Giovanni in PDF and/or ePUB format, as well as other popular books in Medicine & Diagnostics Imaging. We have over 1.5 million books available in our catalogue for you to explore.