Freshney's Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications

Amanda Capes-Davis, R. Ian Freshney

• Publisher: Wiley-Blackwell
• Year: 2021
• Print ISBN: 9781119513018
• eBook ISBN: 9781119513032
• Edition: 8
• Topic: Biological Sciences
• Subtopic: Cell Biology
• Index: Biological Sciences

FRESHNEY'S CULTURE OF ANIMAL CELLS

THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES

Freshney's Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more.

The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text:

Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field

Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting

Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation

Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print

The eighth edition of Freshney's Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Table of contents

• Cover
• Title Page
• Copyright
• Contents
• Foreword
• Acknowledgments
• Abbreviations
• Book Navigation
• Chapter 1 Introduction
• Chapter 2 Biology of Cultured Cells
• Chapter 3 Origin and Evolution of Cultured Cells
• Chapter 4 Laboratory Design And Layout
• Chapter 5 Equipment And Materials
• Chapter 6 Safety And Bioethics
• Chapter 7 Reproducibility And Good Cell Culture Practice
• Chapter 8 Culture Vessels And Substrates
• Chapter 9 Defined Media And Supplements
• Chapter 10 Serum‐Free Media
• Chapter 11 Preparation And Sterilization
• Chapter 12 Aseptic Technique
• Chapter 13 Primary Culture
• Chapter 14 Subculture And Cell Lines
• Chapter 15 Cryopreservation And Banking
• Chapter 16 Microbial Contamination
• Chapter 17 Cell Line Misidentification And Authentication
• Chapter 18 Cell Line Characterization
• Chapter 19 Quantitation And Growth Kinetics
• Chapter 20 Cell Cloning And Selection
• Chapter 21 Cell Separation And Sorting
• Chapter 22 Genetic Modification And Immortalization
• Chapter 23 Culture Of Stem Cells
• Chapter 24 Culture Of Specific Cell Types
• Chapter 25 Culture Of Tumor Cells
• Chapter 26 Differentiation
• Chapter 27 Three‐Dimensional Culture
• Chapter 28 Scale‐Up And Automation
• Chapter 29 Toxicity Testing
• Chapter 30 Training
• Chapter 31 Problem Solving
• Chapter 32 In Conclusion
• Appendix A Glossary
• Appendix B Calculations And Preparation Of Reagents
• Appendix C Media Formulations
• Index
• Eula