Recombinant DNA Principles and Methodologies
eBook - ePub

Recombinant DNA Principles and Methodologies

  1. 768 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Recombinant DNA Principles and Methodologies

About this book

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

  • plasmid biology
  • nucleic acid biochemistry
  • restriction enzymes
  • cloning strategies
  • gel electrophoresis
  • southern and northern blotting
  • preparation of probes
  • phage lambda biology
  • cosmids and genome analysis
  • cloned gene expression
  • polymerase chain reaction
  • conventional and automated DNA sequencing
  • site-directed mutagenesis
  • and more!
    Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
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    Yes, you can access Recombinant DNA Principles and Methodologies by James Greene in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biology. We have over one million books available in our catalogue for you to explore.

    Information

    Table of contents

    1. Cover
    2. Half Title
    3. Title Page
    4. Copyright Page
    5. Preface
    6. Table of Contents
    7. Contributors
    8. 1. Why Cloning?
    9. 2. Biochemistry of Nucleic Acids
    10. 3. Enzymology of Recombinant DNA
    11. 4. Basic Eschericha coli Genetics Useful for Recombinant DNA Techniques
    12. 5. Formulation of Cloning Strategies
    13. 6. Isolation and Purification of Nucleic Acids
    14. 7. Gel Electrophoresis, Southern and Northern Analysis
    15. 8. Hybridization Probes
    16. 9. cDNA Cloning and the Construction of Recombinant DNA
    17. 10. Molecular Cloning by Plasmid Vectors
    18. 11. Phage Lambda: Basic Biology and Use as a Vector
    19. 12. Cosmid Cloning
    20. 13. Multipurpose Cloning Vectors
    21. 14. Mammalian Expression Vectors
    22. 15. Approaches to the Expression of Foreign Genes
    23. 16. Screening for Specific Recombinant Clones
    24. 17. Principles and Applications of the Polymerase Chain Reaction
    25. 18. DNA Sequencing
    26. 19. Site-Directed Mutagenesis
    27. Index