Molecular Analyses

Scott Orland Rogers

• Publisher: CRC Press
• Year: 2022
• Print ISBN: 9780367903718
• eBook ISBN: 9781000596410
• Edition: 1
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership.

Key Features

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• Summarizes nucleic acid extractions from a wide variety of tissues and cells

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• Describes processes of nucleic acid preservation

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• Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms

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• Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses

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• Includes additional methods useful for analysis of nucleic acids and proteins

Related Titles

DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7).

Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9).

Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9)

Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)

Table of contents

• Cover Page
• Half-Title Page
• Series Page
• Title Page
• Copyright Page
• Contents
• Preface
• List of Contributors
• Chapter 1 Nucleic Acid Extraction from Diverse Samples
• Chapter 2 DNA Extraction from Mummified Tissues
• Chapter 3 Commercial DNA Extraction Kits
• Chapter 4 Automated Nucleic Acid Extraction
• Chapter 5 Forensic DNA Samples: Collection and Handling
• Chapter 6 DNA Preservation
• Chapter 7 RNA Storage
• Chapter 8 Disposable Electrochemical DNA Biosensors
• Chapter 9 Handheld Nucleic Acid Analyzer
• Chapter 10 DNA-Binding Fluorophores
• Chapter 11 Fluorescence Resonance Energy Transfer (FRET)
• Chapter 12 LightUp® Probes
• Chapter 13 Invader® Assay
• Chapter 14 Laser-Capture Microdissection
• Chapter 15 Helper-Dependent Adenoviral Vectors
• Chapter 16 Retroviral Vectors
• Chapter 17 Liposomal Nonviral Delivery Vehicles
• Chapter 18 Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing
• Chapter 19 Automated DNA Sequencing
• Chapter 20 DNA Sequencing Methods
• Chapter 21 Differential Sequencing by Mass Spectrometry
• Chapter 22 Brief Guide to Conducting Biological Database Searches
• Chapter 23 Accessing Genomic Databases
• Chapter 24 Phylogenetics, Comparative Genomics, and Phylogenomics
• Chapter 25 DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences
• Chapter 26 ESTAnnotator: A Tool for High-Throughput EST Annotation
• Chapter 27 The Basics of Omics
• Chapter 28 Ribotyping
• Chapter 29 Forensic DNA Typing: Y Chromosome
• Chapter 30 Forensic Identification: An Overview on Molecular Diagnostic Technology
• Chapter 31 Denaturing Gradient Gel Electrophoresis (DGGE)
• Chapter 32 Heteroduplex Analysis (HA)
• Chapter 33 NEBcutter: A Program to Cleave DNA with Restriction Enzymes
• Chapter 34 Differential Display (DD) Analysis
• Chapter 35 Protein Truncation Test (PTT)
• Index