RNA Nanotechnology and Therapeutics

Peixuan Guo, Kirill A. Afonin

• Publisher: CRC Press
• Year: 2022
• Print ISBN: 9781138312869
• eBook ISBN: 9781000552737
• Edition: 2
• Topic: Medicine
• Subtopic: Pharmacology

Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, immunorecognition of RNA nanomaterials, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.

• Presents the latest research and discoveries in RNA nanotechnology
• Features contributions from world-class experts in the field
• Covers RNA nanoparticles in therapeutics
• Describes self-assembled RNA nanoparticles

Table of contents

• Cover Page
• Half Title page
• Title Page
• Copyright Page
• Contents
• Preface
• Acknowledgments
• Introduction
• Contributors
• 1 RNA Structure and Folding
• 2 RNA Regulation and Function in Nature
• 3 Principles and Fundamentals of RNA Nanotechnology
• 4 Computation and Folding Predictions
• 5 Enzymatic Synthesis and Modification of RNA Nanoparticles
• 6 Synthetic and Enzymatic Methods for RNA Labeling and Modifications
• 7 Methods and Assembly of RNA Nanotechnology
• 8 Purification, Characterization, and Structure Determination of RNA Nanoparticles
• 9 SELEX
• 10 Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology
• 11 The Natural Versatility of RNA
• 12 Nucleic Acids as a Building Material in Nanotechnology
• 13 Multiple Functionalities for RNA Nanoparticles
• 14 From Computational RNA Structure Prediction to the Design of Biologically Active RNA-Based Nanostructures
• 15 Application of RNA Tertiary Structure Prediction Tool iFoldRNA in RNA Nanotechnology
• 16 HyperFold A Web Server for Predicting Nucleic Acid Complexes
• 17 RNA Switches Towards Conditional Dynamic RNA-Based Constructs for Therapeutics and Bioassays
• 18 RNA Multiway Junction Motifs as Lego for the Construction of Multifunctional RNA Nanoparticles
• 19 Fabrication Methods for RNA Nanoparticle Assembly Based on Bacteriophage Phi29 pRNA Structural Features
• 20 Purification of RNA, Modified Oligos, and RNA Nanoparticles
• 21 Physicochemical Characterization of Nucleic Acid Nanoparticles
• 22 Light Scattering Techniques for Characterization of NANPs and Their Formulations
• 23 Electron Microscopy of Nucleic Acid Nanoparticles
• 24 A Single-Molecule FRET Approach for Investigating the Binding Mechanisms of Anti-Viral Aptamers
• 25 Entropy and Enthalpy in RNA Nanoparticle Assembly and Thermodynamic Stability for Medical Application Using RNA Nanotechnology
• 26 Immunorecognition of Nucleic Acid Nanoparticles
• 27 Viral Noncoding RNAs in Modulating Cellular Defense and Their Potential for RNA Nanotechnology
• 28 RIG-I as a Therapeutic Target for Nucleic Acid Nanoparticles (NANPs)
• 29 Driving Dynamic Functions with Programmable RNA Nanostructures
• 30 Defining Immunological Properties of Nucleic Acid Nanoparticles Using Human Peripheral Blood Mononuclear Cells
• 31 The Emerging Field of RNA Nanotechnology
• 32 Thermodynamically Stable RNA Three-Way Junction for Constructing Multifunctional Nanoparticles for Delivery of Therapeutics1
• 33 RNAi Nanotherapeutics for Localized Cancer Therapy
• 34 Delivery of RNA Nanoparticles
• 35 Recommendations for Planning In Vivo Studies for RNAi Therapeutics
• 36 Mesoporous Silica Nanoparticles for Efficient siRNA Delivery
• 37 Method of Large-Scale Exosome Purification and Its Use for Pharmaceutical Applications
• 38 Engineered Extracellular Vesicle-Based Therapeutics for Liver Cancer
• 39 Extracellular Vesicles (EVs) An Innovative Approach to Engineering Nucleic Acid Delivery
• 40 Extracellular Vesicles (EVs) Naturally Occurring Vehicles for RNA Nanotherapeutics
• 41 Harnessing Exosomes and Bioinspired Exosome-Like Nanoparticles for siRNA Delivery
• 42 RNA Structural Modeling for Therapeutic Applications
• 43 RNA Micelles for Therapeutics Delivery and Cancer Therapy
• 44 Bacteriophage RNA Leading the Way in RNA Nanotechnology for Targeted Cancer Therapy
• 45 Current State in the Development of RNAi Self-Assembled Nanostructures
• 46 RNA-Based Devices for Diagnostic and Biosensing
• 47 MicroRNAs Biology and Role in RNA Nanotechnology
• 48 Conjugation of RNA Aptamer to RNA Nanoparticles for Targeted Drug Delivery
• 49 MicroRNAs in Human Cancers and Therapeutic Applications
• 50 Tuning the Size, Shape, and Structure of RNA Nanoparticles for Favorable Cancer Targeting and Immunostimulation1
• 51 RNA Nanotechnology and Extracellular Vesicles (EVs) for Gene Therapy
• 52 Application of RNA Aptamers in Nanotechnology and Therapeutics
• Index