
Plant Transformation via Agrobacterium Tumefaciens
Culture Conditions, Recalcitrance and Advances in Soybean
- 226 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
Plant Transformation via Agrobacterium Tumefaciens
Culture Conditions, Recalcitrance and Advances in Soybean
About this book
Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes.
This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment.
Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.
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Information
Table of contents
- Cover
- Half-Title
- Title
- Copyright
- Dedication
- Contents
- Preface
- Acknowledgements
- Author
- Chapter 1 An Overview of Genetic Transformation in Plants
- Chapter 2 Plant Transformation History
- Chapter 3 Agrobacterium tumefaciens
- Chapter 4 Getting Started with in Vitro Soybean Transformation
- Chapter 5 In Vitro Cultures Commonly Used for Plant Transformation
- Chapter 6 Current and Longstanding Challenges Facing Soybean Transformation
- Chapter 7 Alternative Techniques for Genetic Manipulations in Soybean
- Chapter 8 Molecular Aspects of Indirect Gene Transfer via A. tumefaciens
- Chapter 9 Genetic Transformation in Other Leguminous Crops
- Chapter 10 Transgenic Plants for Biotic Stress Resistance in Soybean
- Chapter 11 Transgenic Plants for Abiotic Stress Resistance in Soybean
- Chapter 12 Potential Health Safety Concerns and Environmental Risks
- Chapter 13 The Regulatory Management of Transgenic Plants
- Chapter 14 Current and Future Prospects in Genetic Engineering
- Chapter 15 Photographic Index of Soybean Transformation Cultures
- Glossary
- Index