Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

Khalid Z. Masoodi, Sameena Maqbool Lone, Rovidha Saba Rasool

• Publisher: Academic Press
• Year: 2020
• Print ISBN:
  9780128244494
• eBook ISBN:
  9780128244500
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Dedication
• Preface
• Foreword
• Acknowledgment
• Introduction
• About the author
• Disclaimer
• Chapter 1: Introduction to molecular biology techniques
• Chapter 2: Agarose gel electrophoresis
• Chapter 3: SDS-polyacrylamide gel electrophoresis
• Chapter 4: Immunoelectrophoresis
• Chapter 5: Plasmid DNA isolation using alkaline lysis method from E. coli DH5α cells
• Chapter 6: Restriction digestion of plasmid DNA
• Chapter 7: Genomic DNA extraction from the plant leaves using the CTAB method
• Chapter 8: Genomic DNA extraction from silkworm using modified phenol:chloroform method (Suzuki et al., 1972)
• Chapter 9: Genomic DNA extraction from Fungal Mycelium using the modified Cenis, 1992 method
• Chapter 10: Bacterial transformation using CaCl2
• Chapter 11: Transformation of E. coli by electroporation
• Chapter 12: Transfection of cells using lipofectamine
• Chapter 13: Total RNA isolation
• Chapter 14: RNA isolation from plant tissues with high phenolic compounds and polysaccharides
• Chapter 15: Total protein extraction with TCA-acetone and 2D gel electrophoresis
• Chapter 16: Dansyl-Edman method of peptide sequencing
• Chapter 17: ISSR analysis
• Chapter 18: Gene cloning/recombinant DNA technology
• Chapter 19: Polymerase chain reaction (PCR)
• Chapter 20: Blotting/hybridization and its types
• Chapter 21: Dot blot analysis
• Chapter 22: ELISA (enzyme-linked immunosorbent assay)
• Chapter 23: Study of principle of centrifugation
• Chapter 24: Study of principle of chromatography
• Chapter 25: Thin layer chromatography (TLC)
• Chapter 26: Gel-filtration or size-exclusion chromatography
• Chapter 27: Ion-exchange chromatography
• Chapter 28: To perform affinity chromatography
• Chapter 29: To perform paper chromatography
• Chapter 30: Growth of bacterial cultures and preparation of growth curve
• Chapter 31: To study standard operating procedures (SOPs) for various equipment
• Index