Advanced Methods in Molecular Biology and Biotechnology
eBook - ePub

Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool

  1. 218 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Advanced Methods in Molecular Biology and Biotechnology

A Practical Lab Manual

Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool

Book details
Table of contents
Citations

About This Book

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.

This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

  • Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
  • Features clear, step-by-step instruction for applying the techniques covered
  • Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

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Information

Publisher
Academic Press
Year
2020
ISBN
9780128244500
Topic
Scienze biologiche
Subtopic
Biochimica

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright
  5. Dedication
  6. Preface
  7. Foreword
  8. Acknowledgment
  9. Introduction
  10. About the author
  11. Disclaimer
  12. Chapter 1: Introduction to molecular biology techniques
  13. Chapter 2: Agarose gel electrophoresis
  14. Chapter 3: SDS-polyacrylamide gel electrophoresis
  15. Chapter 4: Immunoelectrophoresis
  16. Chapter 5: Plasmid DNA isolation using alkaline lysis method from E. coli DH5α cells
  17. Chapter 6: Restriction digestion of plasmid DNA
  18. Chapter 7: Genomic DNA extraction from the plant leaves using the CTAB method
  19. Chapter 8: Genomic DNA extraction from silkworm using modified phenol:chloroform method (Suzuki et al., 1972)
  20. Chapter 9: Genomic DNA extraction from Fungal Mycelium using the modified Cenis, 1992 method
  21. Chapter 10: Bacterial transformation using CaCl2
  22. Chapter 11: Transformation of E. coli by electroporation
  23. Chapter 12: Transfection of cells using lipofectamine
  24. Chapter 13: Total RNA isolation
  25. Chapter 14: RNA isolation from plant tissues with high phenolic compounds and polysaccharides
  26. Chapter 15: Total protein extraction with TCA-acetone and 2D gel electrophoresis
  27. Chapter 16: Dansyl-Edman method of peptide sequencing
  28. Chapter 17: ISSR analysis
  29. Chapter 18: Gene cloning/recombinant DNA technology
  30. Chapter 19: Polymerase chain reaction (PCR)
  31. Chapter 20: Blotting/hybridization and its types
  32. Chapter 21: Dot blot analysis
  33. Chapter 22: ELISA (enzyme-linked immunosorbent assay)
  34. Chapter 23: Study of principle of centrifugation
  35. Chapter 24: Study of principle of chromatography
  36. Chapter 25: Thin layer chromatography (TLC)
  37. Chapter 26: Gel-filtration or size-exclusion chromatography
  38. Chapter 27: Ion-exchange chromatography
  39. Chapter 28: To perform affinity chromatography
  40. Chapter 29: To perform paper chromatography
  41. Chapter 30: Growth of bacterial cultures and preparation of growth curve
  42. Chapter 31: To study standard operating procedures (SOPs) for various equipment
  43. Index