
- 240 pages
- English
- PDF
- Available on iOS & Android
Laboratory Exercises and Techniques in Cellular Biology
About this book
The Contento Experimental Cell Biology Lab Book is a modular design that matches the topics discussed in Karp's textbook. The manual itself consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text. There are three possible designs of the lab book, based on the instructor's needs. These designs focus on either Techniques, Concepts, or Organelles. The procedures of the 30+ experiments remain standard and unchanged in all designs of the lab book. Special Overview pages, Discussion Questions and Datasheets bookend the procedures in order to create each of the possible textbook designs.This gives instructors flexibility to create a lab book that suits their lecture course curriculum, their experience, and available equipment and supplies.
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Information
Table of contents
- Cover
- Title Page
- Copyright Page
- Contents
- Introduction
- Acknowledgments
- Overview 1: Introduction and Microscopy
- Overview 2: Enzyme Kinetics
- Overview 3: Membrane Permeability
- Overview 4: Protein Transport to the Plasma Membrane
- Overview 5: Cell Fractionation
- Overview 6: Isolation and Activity of Mitochondria
- Overview 7: Staining of the Extracellular Space
- Overview 8: Protoplast Isolation and Vacuole Staining
- Overview 9: Isolation of Vacuoles
- Overview 10: Staining of Autophagosomes and Lysosomes
- Overview 11: Staining of the Cytoskeleton
- Overview 12: Cell Motility
- Overview 13: Staining of the Nucleus and Nucleic Acids
- Overview 14: Staining of Chromosome and Cell Cycle Analysis
- Overview 15: Staining for Cell Viability and Apoptosis
- Overview 16: Staining of Immune Cells
- Overview 17: Protein Isolation, Fractionation, and SDS-PAGE
- Overview 18: Genomic DNA Isolation, Purification, and the Polymerase Chain Reaction
- Overview 19: Ligation and Cloning
- Overview 20: Restriction Fragment Length Polymorphism Analysis and Agarose Gel Electrophoresis
- Overview 21: Staining of Peroxisomes
- Overview 22: Short-Term Aggregation Culture
- Overview 23: Staining of the Endoplasmic Reticulum and the Golgi Apparatus
- Overview 24: The Endocytic Pathway (FM4-64 Staining)
- Overview 25: Detection of MAP Kinase Signaling
- Overview 26: Cancer
- Overview 27: Western Blot
- Overview 28: Enzyme-Linked Immunosorbent Assay
- Overview 29: Southern and Northern Blotting
- Overview 30: Using Online Research Tools
- Index
- Instructor's Notes