Droplets of Life

Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid-Liquid Phase Separation

Vladimir N Uversky, Vladimir N. Uversky

• Publisher: Academic Press
• Year: 2022
• Print ISBN: 9780128239674
• eBook ISBN: 9780128241752
• Topic: Biological Sciences
• Subtopic: Cell Biology
• Index: Biological Sciences

Droplets of Life: Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid–Liquid Phase Separation provides foundational information on the biophysics, biogenesis, structure, functions, and roles of membrane-less organelles. The study of liquid–liquid phase separation has attracted a lot of attention from disciplines such as cell biology, biophysics, biochemistry, and others trying to understand how, why, and what roles these condensates play in homeostasis and disease states in living organisms. This book's editor recruited a group of international experts to provide a current and authoritative overview of all aspects associated with this exciting area.Sections introduce membrane-less organelles (MLOs) and biomolecular condensates; MLOs in different sizes, shapes, and composition; and the formation of MLOs due to phase separation and how it can tune reactions, organize the intracellular environment, and provide a role in cellular fitness. . - Presents the first book to establish the foundations of this exciting research area - Combines biophysics, structural and cell biology, and biochemistry perspectives into a single volume - Edited and authored by world-leading scientists - Covers basic physical and biological principles and health and disease implications

Table of contents

• Droplets of Life
• Cover
• Title Page
• Copyright
• Table of Contents
• Contributors
• Chapter 1 Biophysical principles of liquid–liquid phase separation
• Chapter 2 Major structural features of membrane-less organelles
• Chapter 3 Biochemical and structural biology aspects of liquid–liquid phase separation: protein side of liquid–liquid phase separation, membrane-less organelles, and biomolecular condensates
• Chapter 4 Biochemical and structural biology aspects of liquid–liquid phase separation: an interplay between proteins and RNA
• Chapter 5 Liquid–liquid phase separation and biomolecular condensates in cell quiescence
• Chapter 6 Multiphase complex droplets
• Chapter 7 Techniques for the detection and analysis of LLPS and MLOs
• Chapter 8 Guidelines for experimental characterization of liquid–liquid phase separation in vitro
• Chapter 9 Liquid–liquid phase separation at the origins of life
• Chapter 10 Known types of membrane-less organelles and biomolecular condensates
• Chapter 11 Active regulation mechanisms of LLPS and MLOs biogenesis
• Chapter 12 Interactions and interplay of MLOs with classical membrane-bound organelles
• Chapter 13 Intrinsically disordered regions: a platform for regulated assembly of biomolecular condensates
• Chapter 14 MLOs and control of metabolic pathways
• Chapter 15 LLPS and regulation of transmembrane signaling
• Chapter 16 Phase separation in chromatin-based intranuclear processes
• Chapter 17 MLOstasis: liquid–liquid phase separation and biomolecular condensates in cell competition, fitness, and aging
• Chapter 18 Stress, membraneless organelles, and liquid–liquid phase separation
• Chapter 19 Roles of phase separation and condensates in autophagy
• Chapter 20 Liquid–liquid phase in anhydrobiosis
• Chapter 21 Plant biomolecular condensates
• Chapter 22 Droplets of life: role of phase separation in virus replication and compartmentalization
• Chapter 23 Liquid–liquid phase separation in neurodegenerative diseases
• Chapter 24 Emerging roles of liquid–liquid phase separation and membraneless organelles in cancer progression
• Chapter 25 Liquid–liquid phase separation, membrane-less organelles, and biomolecular condensates in cardiovascular disease
• Chapter 26 Phase separation and infectious diseases
• Index