RNA Methodologies

A Laboratory Guide for Isolation and Characterization

Robert E. Farrell Jr., Robert E. Farrell, Jr.

• Publisher: Academic Press
• Year: 2022
• eBook ISBN: 9780323916561
• Edition: 6
• Topic: Biological Sciences
• Subtopic: Cell Biology
• Index: Biological Sciences

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. - Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology - Builds from basic information on RNA techniques to in-depth protocols for specific applications - Features new chapters on RNA sequencing and RNA in situ hybridization - Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus - Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics

Table of contents

• RNA Methodologies
• Chapter 1 RNA and the cellular biochemistry revisited
• Chapter 2 Creating a ribonuclease-free environment
• Chapter 3 Stringency: conditions that influence nucleic acid structure
• Chapter 4 RNA isolation strategies
• Chapter 5 Isolation of polyadenylated RNA
• Chapter 6 The truth about tissues
• Chapter 7 Going green: RNA and the molecular biology of plants
• Chapter 8 Quality control for RNA preparations
• Chapter 9 cDNA: a permanent biochemical record of the cell
• Chapter 10 RT-PCR: a science and an art form
• Chapter 11 Quantitative PCR techniques
• Chapter 12 miRNA and other noncoding RNAs
• Chapter 13 RNA interference and gene editing
• Chapter 14 Electrophoresis of RNA
• Chapter 15 Photodocumentation and image analysis
• Chapter 16 Northern analysis
• Chapter 17 Nucleic acid probe technology
• Chapter 18 Quantification of specific mRNAs by nuclease protection
• Chapter 19 Analysis of nuclear RNA
• Chapter 20 RNA in situ hybridization
• Chapter 21 Array analysis of gene expression
• Chapter 22 Subtractive and nonsubtractive methods for the analysis of gene expression
• Chapter 23 Transcriptomes and bioinformatics
• Chapter 24 RNA-seq: the premier transcriptomics tool
• Chapter 25 RNA biomarker discovery and validation
• Chapter 26 Functional genomics strategies
• Chapter 27 A few RNA success stories
• Epilogue
• Appendix A Maintaining complete and accurate records
• Appendix B Converting mass to moles
• Appendix C Removal of DNA from an RNA sample
• Appendix D Removal of RNA from a DNA sample
• Appendix E Electrophoresis: principles, parameters, and safety
• Appendix F Disposal of ethidium bromide and SYBR Green solutions
• Appendix G Deionization of formamide, formaldehyde, and glyoxal
• Appendix H Silanizing centrifuge tubes and glassware
• Appendix I Centrifugation as a mainstream tool for the molecular biologist
• Appendix J Dot blot analysis
• Appendix K Useful stock solutions for the molecular biologist
• Appendix L Genomes and proteomes
• Appendix M Common SI prefixes
• Appendix N Common abbreviations
• Appendix O Select suppliers of equipment, reagents, and services
• Appendix P Trademark citations
• Index