Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource. - Provides a practical resource on designing, executing and analyzing experiments and analytical procedures - Includes detailed and standardized coverage of basic research methods in the area - Presents step-by-step instructions on how to execute a large selection of life sciences experiments

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Yes, you can access Basic Life Science Methods by Javeed Ahmad Tantray,Nighat Un Nissa,Rasy Fayaz Choh Wani,Sheikh Mansoor Shafi,Sheikh Mansoor in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biochemistry. We have over one million books available in our catalogue for you to explore.

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Index

Title of Book
Cover image
Title page
Table of Contents
Copyright
List of figures
List of tables
About the authors
Foreword
Foreword
Introduction
Chapter 1 Calculations of molarity and normality
Chapter 2 Glassware washing and sterilization techniques
Chapter 3 pH meter: Its use and calibration
Chapter 4 Principle and working of autoclave
Chapter 5 Principle and working of centrifuge
Chapter 6 Principle and working of laminar
Chapter 7 Estimation of total cholesterol (Flegg, 1972)
Chapter 8 Estimation of creatinine
Chapter 9 Estimation of blood urea
Chapter 10 Estimation of blood glucose by GOD—POD method
Chapter 11 Determination of albumin and globulin in plasma
Chapter 12 Quantitative determination of homocysteine
Chapter 13 Estimation of serum bilirubin
Chapter 14 Estimation of diagnostic enzyme ALT
Chapter 15 Estimation of diagnostic enzyme AST
Chapter 16 Protein estimation by Lowry's method
Chapter 17 Estimation of reducing sugar by using dinitro salicylic acid method
Chapter 18 To perform quantitative determination of total protein in serum/plasma
Chapter 19 Enzyme activity using starch assay
Chapter 20 To estimate protein by Bradford assay
Chapter 21 To identify a protein sample using SDS-PAGE
Chapter 22 Genomic DNA isolation from whole blood
Chapter 23 PCR for gene amplification
Chapter 24 Agarose gel electrophoresis
Chapter 25 RNA isolation from human tissue
Chapter 26 Real-time polymerase chain reaction
Chapter 27 Preparation of competent cells by CaCl2 treatment
Chapter 28 RNA isolation from plant tissues
Chapter 29 DNA isolation from the bacterial culture broth
Chapter 30 DNA isolation from fungal mycelium
Chapter 31 Quantification of the DNA using spectrophotometer
Chapter 32 Formaldehyde gel electrophoresis of the isolated RNA sample
Chapter 33 Paper chromatography of different amino acid
Chapter 34 Separation of plant pigments using column chromatography
Chapter 35 Separation of plant pigment by thin-layer chromatography
Chapter 36 Chromatin immunoprecipitation protocol
Chapter 37 Types of media
Chapter 38 Preparation of nutrient agar media
Chapter 39 Serial dilution of the bacterial cells
Chapter 40 Spread plate method of the bacterial cells
Chapter 41 Streak plate method
Chapter 42 Identified bacterial colony in culture broth
Chapter 43 Pour plate method for bacterial colony counting
Chapter 44 Gram staining of bacteria
Chapter 45 Casein hydrolysis test
Chapter 46 Antibiotic resistance test by agar well diffusion method
Index

About this book

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Table of contents