Gene Cloning and Analysis
eBook - ePub
Available until 21 Jul |Learn more

Gene Cloning and Analysis

Current Innovations

B.C. Schaefer

  1. 212 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub
Available until 21 Jul |Learn more

Gene Cloning and Analysis

Current Innovations

B.C. Schaefer

Book details
Table of contents
Citations

About This Book

This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic

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Information

Publisher
Garland Science
Year
2023
ISBN
9781000950083
Edition
1
Topic
Biological Sciences
Subtopic
Biochemistry
Index
Biological Sciences

Table of contents

  1. Cover
  2. Title Page
  3. Copyright Page
  4. Preface
  5. Table of Contents
  6. List the Contributors
  7. Chapter 1: Adjustment of Parameters in the Yeast Two-Hybrid System: Criteria for Detecting Physiologically Significant Protein-Protein Interactions
  8. Chapter 2: pJuFo: A Phage Surface Display System for Cloning Genes Based on Protein-ligand Interaction
  9. Chapter 3: Restriction Endonuclease-based Gene Expression Fingerprinting
  10. Chapter 4: PNA Used for Specific Capture of Nucleic Acids
  11. Chapter 5: A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence
  12. Chapter 6: Affinity Selection of Eukaryotic mRNAs And Full-length cDNAs by a CAP Retention Procedure (CAPture)
  13. Chapter 7: RNA Ligase-mediated Race: An Effective Method for the Cloning of Full-length cDNA Ends
  14. Chapter 8: Combinatorial Scanning Site-directed Mutagenesis
  15. Chapter 9: A Rapid Method for Creating Gene Mutations and Fusions Using PCR-ligation-PCR Mutagenesis
  16. Chapter 10: Double Replacement Gene Targeting in Embryonic Stem Cells for the Introduction of Subtle Alterations into Endogenous Mouse Genes
  17. Chapter 11: Universal TA Cloning
  18. Chapter 12: Long PCR: Rapid Restriction Mapping and Selective Suppression by Restriction Digestion
  19. Chapter 13: High-resolution Silver Staining-based PCR-single Strand Conformation Polymorphism (SSCP) Analysis
  20. Index