Clinical Biochemistry

A Laboratory Guide

Rooma Devi, Aman Chauhan, Simmi Kharb, Chandra Shekhar Pundir

• Publisher: Jenny Stanford Publishing
• Year: 2023
• ISBN:
  9781000988949
• Edition: 1
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

A knowledge of the practical aspects of clinical biochemistry is essential for medical students to understand the diagnostic and prognostic status of any ailment. This book addresses and compiles some of the invariably used protocols in biochemistry with the aim to enhance the understanding of the basics behind following the methodologies, including those for urine analysis; carbohydrate, protein, and lipid analyses; and diabetes and lipid profile. It discusses organ function tests, such as liver, renal, or pancreatic function tests, in addition to exploring pH and buffer, colorimeter, spectrophotometer, chromatography, electrophoresis, enzyme-linked adsorbent assay, radioimmunoassay, biosensor/glucometer, fructosamine, DNA isolation, mass spectrometry, flow cytometry, centrifugation, automation, cerebrospinal fluid examination, and oxalic acid test. The book also touches upon the basics of laboratory safety rules and laboratory glassware.

The book is helpful for students to understand the relationship between quality management and specimen collection and processing them for their biological functions. It is well-suited for academic courses, including undergraduate, graduate, postgraduate, and professional courses and covers the practical syllabus for MBBS, BDS, BSc MLT, BSc nursing, and MSc students. For undergraduate students in the disciplines of biochemistry, molecular biology, chemistry, and other related biological sciences, the book forms an integral part of the syllabus with its case studies, MCQs, biochemistry, OSPEs, etc. It also demonstrates the actual experiments for understanding the concepts better and is a must read for students to acquire skills to work with a clinical biochemistry laboratory.

Table of contents

• Cover Page
• Half Title page
• Title Page
• Copyright Page
• Contents
• Preface
• 1. Lab Safety Rules
• 2. Specimen Collection and Processing
• 3. Quality Management in the Laboratory
• 4. Urine Analysis
• 5. Carbohydrates
• 6. Blood Glucose
• 7. Proteins
• 8. Lipid Analysis
• 9. Lipid Profile
• 10. Liver Function Test
• 11. Renal Function Tests
• 12. Pancreatic Function Test
• 13. Acids and Bases
• 14. Colorimetry
• 15. Chromatography
• 16. Electrophoresis
• 17. Enzyme-Linked Immunosorbent Assay
• 18. Radioimmunoassay
• 19. Polymerase Chain Reaction
• 20. Biosensors/Glucometer
• 21. Glycated Hemoglobin
• 22. Enzymes
• 23. DNA Isolation from Blood and Tissue
• 24. Mass Spectrometry
• 25. Flow Cytometry
• 26. Centrifugation
• 27. Automation
• 28. Cerebrospinal Fluid Examination
• 29. Oxalic Acid
• Index