Manual of Sperm Function Testing in Human Assisted Reproduction
eBook - PDF

Manual of Sperm Function Testing in Human Assisted Reproduction

  1. 288 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Manual of Sperm Function Testing in Human Assisted Reproduction

About this book

Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men.

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Information

Publisher
Cambridge University Press
Year
2021
Print ISBN
9781108793537
eBook ISBN
9781108881890
Topic
Medicine
Subtopic
Gynecology, Obstetrics & Midwifery

Table of contents

  1. Cover
  2. Half-title
  3. Series information
  4. Title page
  5. Copyright information
  6. Dedication
  7. Contents
  8. List of Contributors
  9. Short Biography
  10. Foreword
  11. Preface
  12. Introduction
  13. Chapter 1 Standard Semen Examination: Manual Semen Analysis
  14. Chapter 2 Standard Semen Analysis: Computer-Assisted Semen Analysis
  15. Chapter 3 Standard Semen Analysis: Home Sperm Testing
  16. Chapter 4 Standard Semen Analysis: Leukocytospermia
  17. Chapter 5 Standard Semen Analysis: Morphology
  18. Chapter 6 Sperm Vitality: Eosin-Nigrosin Dye Exclusion
  19. Chapter 7 Sperm Vitality: Hypo-Osmotic Swelling Test
  20. Chapter 8 Determination of Mitochondrial Membrane Potential by Flow Cytometry in Human Sperm Cells
  21. Chapter 9 Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction
  22. Chapter 10 Capacitation and Acrosome Reaction: Histochemical Techniques to Determine Acrosome Reaction
  23. Chapter 11 Zona Binding: Competitive Sperm-Binding Assay
  24. Chapter 12 Zona Binding: Hemizona Assay
  25. Chapter 13 Oolemma Binding: Sperm Penetration Assay
  26. Chapter 14 Oxidative Stress Testing: Direct Tests
  27. Chapter 15 Oxidative Stress Testing: Indirect Tests
  28. Chapter 16 Chromatin Condensation: Aniline Blue Stain
  29. Chapter 17 Chromatin Condensation: Chromomycin A3 (CMA3) Stain
  30. Chapter 18 Sperm Chromatin Structure: Toluidine Blue Staining
  31. Chapter 19 DNA Damage: TdT-Mediated dUTP Nick-End-Labelling Assay
  32. Chapter 20 DNA Damage: Sperm Chromatin Structure Assay: Sperm Chromatin Structure Assay Test on its Fortieth Anniversary
  33. Chapter 21 DNA Damage: COMET Assay
  34. Chapter 22 DNA Damage: Halo Sperm Test
  35. Chapter 23 DNA Damage: Fluorescent In-Situ Hybridization
  36. Chapter 24 Clinical Value of Sperm Function Tests
  37. Chapter 25 Future Developments: Sperm Proteomics
  38. Conclusion
  39. Index

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Yes, you can access Manual of Sperm Function Testing in Human Assisted Reproduction by Ashok Agarwal,Ralf Henkel,Ahmad Majzoub in PDF and/or ePUB format, as well as other popular books in Medicine & Gynecology, Obstetrics & Midwifery. We have over 1.5 million books available in our catalogue for you to explore.