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About this book
The membrane is an intricately structured entity that performs numerous vital biological functions, including materials transport, signal transduction, energy transfer, and enzymatic reactions. Abnormal expression and distribution of certain membrane proteins are even associated with genetic disorders, neurodegenerative diseases, and malignant tumors. Despite the widely acknowledged significance of membranes, comprehensive and systematic research on membranes akin to genomics, proteomics, and metabolomics is still lacking. Furthermore, the broad concept of biomembranes is not confined to the plasma membrane alone; it also includes organelle membranes and membranes of endocytosis or exocytosis vesicles, which are also derived from the biomembrane system.
This book introduces the concept of "omics" to membranes and proposes the term 'biomembranomics.' It compiles the latest advancements in structural analysis techniques for biomembranes, including single-molecule manipulation techniques, single-molecule fluorescence techniques, super-resolution fluorescence imaging, cryo-electron microscopy, mass spectrometry, molecular dynamics simulation, and hyphenated instrumental techniques. The book presents both classic and cutting-edge protocols in text and illustrative forms, serving as a valuable and applicable reference material. It provides a profound understanding of biomembrane organization at single-molecule level, paving new avenues for unveiling the relationship between membrane structure and function. Therefore, this book is essential reading for researchers across all related fields.
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Table of contents
- Cover Page
- Half Title Page
- Title Page
- Copyright Page
- Contents
- Preface
- Acknowledgements
- Chapter 1 Biomembranomics: New Concept
- Chapter 2 Mass Spectrometry for Studying Ensemble Biomembranomics
- Chapter 3 Mapping Membrane by High-Resolution Atomic Force Microscopy
- Chapter 4 Super-Resolution Imaging for Mapping Membrane Proteins and Carbohydrates
- Chapter 5 Fluorescence Microscopy for Studying Plasma Membrane and Intracellular Membranes
- Chapter 6 Single-Molecule Fluorescence for Studying the Membrane Protein Dynamics and Interactions
- Chapter 7 Cryo-Transmission Electron Microscopy for Studying Cell Membranes
- Chapter 8 Cryo-Scanning Electron Microscope for Studying Plasma Membranes
- Chapter 9 Infrared Spectroscopy Studying Plasma Membrane
- Chapter 10 Application of Single-Molecule Force Spectroscopy in Membrane Receptors Dynamics and Signal Transduction
- Chapter 11 Studying Membrane Dynamics Using Force Spectroscopy Based on Atomic Force Microscopy
- Chapter 12 Computer Simulations to Explore Membrane Organization and Transport
- Index