Bio-Nano Interfaces

Perspectives, Properties, and Applications

Wolfgang Parak

• Publisher: Jenny Stanford Publishing
• Year: 2024
• Print ISBN: 9789814877831
• eBook ISBN: 9781000618921
• Edition: 1
• Topic: Technology & Engineering
• Subtopic: Biotechnology in Medicine
• Index: Technology & Engineering

In recent decades, bio-nano interfaces have become a popular topic of research. The interface between biology (e.g., cells, proteins) and man-made materials (e.g., surfaces of labware, medical devices/implants, etc., that are exposed to the biological matter) has always been important, way before the terms of nanotechnology and nanoscience were coined. Nanotechnology brought new techniques into play, with which such interfaces can be investigated with an additional viewpoint.

This book is a collection of articles spanning two decades that shows how the newer publications have evolved from the older ones. This allows the reader to see the development in the field not only technically but also conceptually. The book is, in particular, suitable for the researchers and general readers who are looking for inspiration on how ideas develop over decades.

Table of contents

• Cover Page
• Half Title page
• Title Page
• Copyright Page
• Contents
• Preface
• Introduction
• 1 Highly Integrated Surface Potential Sensors
• 2 Lateral Resolution of Light-addressable Potentiometric Sensors: An Experimental and Theoretical Investigation
• 3 Metabolic Activation Stimulates Acid Production in Synovial Fibroblasts
• 4 Spatially Resolved Monitoring of Cellular Metabolic Activity with a Semiconductor-Based Biosensor
• 5 Extracellular Measurements of Averaged Ionic Currents with the Light-Addressable Potentiometric Sensor (LAPS)
• 6 Quantum Dots on Gold: Electrodes for Photoswitchable Cytochrome c Electrochemistry
• 7 Immobilization of Quantum Dots via Conjugated Self-Assembled Monolayers and Their Application as a Light-Controlled Sensor for the Detection of Hydrogen Peroxide
• 8 Multiplexed Readout of Enzymatic Reactions by Means of Laterally Resolved Illumination of Quantum Dot Electrodes
• 9 Corrosion Protection and Long-Term Chemical Functionalization of Gallium Arsenide in an Aqueous Environment
• 10 Cytotoxicity of Colloidal CdSe and CdSe/ZnS Nanoparticles
• 11 The Toxicity of Silver Nanoparticles Depends on Their Uptake by Cells and Thus on Their Surface Chemistry
• 12 Investigation of the Viability of Cells upon Co-Exposure to Gold and Iron Oxide Nanoparticles
• Colloidal Gold Nanoparticles Induce Changes in Cellular and Subcellular Morphology
• 14 Conjugation of DNA to Silanized Colloidal Semiconductor Nanocrystalline Quantum Dots
• 15 Conformation of Oligonucleotides Attached to Gold Nanocrystals Probed by Gel Electrophoresis
• 16 Hydrophobic Nanocrystals Coated with an Amphiphilic Polymer Shell: A General Route to Water Soluble Nanocrystals
• 17 Design of an Amphiphilic Polymer for Nanoparticle Coating and Functionalization
• 18 Multifunctional Nanoparticles for Dual Imaging
• 19 Electrophoretic Separation of Nanoparticles with a Discrete Number of Functional Groups
• 20 Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion
• 21 Tumour Homing and Therapeutic Effect of Colloidal Nanoparticles Depend on the Number of Attached Antibodies
• 22 Molecular Weight, Osmotic Second Virial Coefficient, and Extinction Coefficient of Colloidal CdSe Nanocrystals
• 23 Size Determination of (Bio)conjugated Water-Soluble Colloidal Nanoparticles: A Comparison of Different Techniques
• 24 Protein Corona Formation around Nanoparticles—From the Past to the Future*
• 25 Ion and pH Sensing with Colloidal Nanoparticles: Influence of Surface Charge on Sensing and Colloidal Properties
• The Challenge to Relate the Physicochemical Properties of Colloidal Nanoparticles to Their Cytotoxicity
• 27 Selected Standard Protocols for the Synthesis, Phase Transfer, and Characterization of Inorganic Colloidal Nanoparticles
• 28 Basic Physicochemical Properties of Polyethylene Glycol Coated Gold Nanoparticles that Determine Their Interaction with Cells
• 29 How Entanglement of Different Physicochemical Properties Complicates the Prediction of in Vitro and in Vivo Interactions of Gold Nanoparticles
• 30 Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles
• 31 A Quantitative Fluorescence Study of Protein Monolayer Formation on Colloidal Nanoparticles
• 32 Polymer-Coated Nanoparticles Interacting with Proteins and Cells: Focusing on the Sign of the Net Charge
• 33 Surface Functionalization of Nanoparticles with Polyethylene Glycol: Effects on Protein Adsorption and Cellular Uptake
• 34 In situ Detection of the Protein Corona in Complex Environments
• 35 Tracking Stem Cells and Macrophages with Gold and Iron Oxide Nanoparticles-the Choice of the Best Suited Particles
• 36 Quantitative Particle-Cell Interaction: Some Basic Physicochemical Pitfalls
• 37 Quantitative Particle Uptake by Cells as Analyzed by Different Methods
• 38 Physicochemical Properties of Protein-Coated Gold Nanoparticles in Biological Fluids and Cells before and after Proteolytic Digestion
• 39 In Vivo Integrity of Polymer-Coated Gold Nanoparticles
• 40 Biodegradation of Bi-Labeled Polymer-Coated Rare-Earth Nanoparticles in Adherent Cell Cultures
• 41 Triple-Labeling of Polymer-Coated Quantum Dots and Adsorbed Proteins for Tracing their Fate in Cell Cultures
• 42 Nanoengineered Polymer Capsules: Tools for Detection, Controlled Delivery, and Site-Specific Manipulation
• 43 Multiple Internalization Pathways of Polyelectrolyte Multilayer Capsules into Mammalian Cells
• 44 Laser-Induced Release of Encapsulated Materials inside Living Cells
• 45 Photoactivated Release of Cargo from the Cavity of Polyelectrolyte Capsules to the Cytosol of Cells
• 46 NIR-Light Triggered Delivery of Macromolecules into the Cytosol
• 47 Light-Addressable Capsules as Caged Compound Matrix for Controlled Triggering of Cytosolic Reactions
• 48 Laterally and Temporally Controlled lntracellular Staining by Light-Triggered Release of Encapsulated Fluorescent Markers
• 49 Control of Wnt/β-Catenin Signaling Pathway In Vivo via Light Responsive Capsules
• 50 Magnetically Triggered Release of Molecular Cargo from Iron Oxide Nanoparticle Loaded Microcapsules
• 51 Polymer Microcapsules as Mobile Local pH-Sensors
• 52 Synthesis and Characterization of Ratiometric Ion-Sensitive Polyelectrolyte Capsules
• 53 Multiplexed Sensing of Ions with Barcoded Polyelectrolyte Capsules
• 54 pH-Sensitive Capsules as Intracellular Optical Reporters for Monitoring Lysosomal pH Changes Upon Stimulation
• 55 Lysosomal Proton Buffering of Poly(ethylenimine) Measured In Situ by Fluorescent pH-Sensor Microcapsules
• 56 Stiffness-Dependent In Vitro Uptake and Lysosomal Acidification of Colloidal Particles
• 57 Multilayer Polyelectrolyte Capsules for Sensing and Drug Delivery: Fundamentals and Applications
• Index