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Light Sheet Fluorescence Microscopy
About this book
Light Sheet Fluorescence Microscopy
An indispensable guide to a novel, revolutionary fluorescence microscopy technique!
Light sheet fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis.
Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a "do-it-yourself" light sheet microscope, making the technique affordable for every laboratory.
This important textbook:
- Serves as an easy-to-understand introduction to light sheet-based fluorescence
- Includes numerous tips and tricks for advanced practitioners
- Provides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab
- Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi
Aimed at cell biologists, biophysicists, developmental biologists, and neuro-biologists, Light Sheet Fluorescence Microscopy offers a comprehensive overview of the most recent applications of this microscopy technique.
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Information
Table of contents
- Cover
- Table of Contents
- Title Page
- Copyright
- Dedication
- Foreword by Ernst H. K. Stelzer
- Preface
- 1 Let There be Light Sheet
- 2 Illumination in Light Sheet Fluorescence Microscopy
- 3 A Small Guide on How to Mount a Sample in a Light-Sheet Microscope
- 4 Detection in a Light Sheet Microscope
- 5 Light Sheet Microscope Configurations
- 6 Commercial and Open-Source Systems
- 7 Image Processing and Analysis of Light Sheet Microscopy Data
- 8 Imaging Molecular Dynamics Using a Light Sheet Microscope
- 9 Light-Sheet Applications: From Rare Cell Detection to Full Organ Analysis
- 10 Single-Objective Light-Sheet Microscopy
- 11 How to Organize a Practical Course on Light Sheet Microscopy
- 12 Light-Sheet Microscopy Technology in the Multiuser Environment of a Core Imaging Facility - Practical Considerations
- Index
- End User License Agreement