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Intracellular Thermometry with Fluorescent Molecular Thermometers
About this book
Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale
Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology.
Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications.
Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find:
- Step by step instructions for constructing an intracellular thermometry experiment and validating results
- Comprehensive discussion of existing applications
- A vision for the future development of thermal biology as an independent discipline
Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.
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Information
Table of contents
- Intracellular Thermometry with Fluorescent Molecular Thermometers
- Preface
- Contents
- 1 Temperature for Living Things
- 2 Fluorescent Molecular Thermometers
- 3 Intracellular Thermometry with Fluorescent Molecular Thermometers
- 4 Cellular Thermometry Based on Non-fluorometric Principles
- 5 Reliability Issue in Intracellular Thermometry
- 6 Applications of Intracellular Thermometry
- Appendix 1 Review and Feature Articles on Fluorescent Molecular Thermometers and Intracellular Thermometry in General
- Appendix 2 Comprehensive Collection of Fluorescent Polymeric Thermometers Based on the Combination of a Thermoresponsive Polymer and an Environment-sensitive Fluorophore
- Appendix 3 Comprehensive Collection of Fluorescent Nanogel Thermometers Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorophore
- Appendix 4 Comprehensive Collection of Non-covalent Fluorescent Temperature Sensing Systems Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorescent Compound
- Appendix 5 Comprehensive Collection of Fluorescent Polymeric Logic Gates Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorophore
- Appendix 6 Solid Inorganic Nanostructures Showing Temperaturedependent Emission Properties
- Appendix 7 Tips for Intracellular Thermometry with Fluorescent Polymeric Thermometers Developed in the Author’s Laboratory
- Index
- EULA