This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products.

Key Features:

Provides step-by-step procedures and troubleshooting tips
Covers identification, growth parameters and pigment estimation methodologies
Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria
Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products
Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria

This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.

Trusted by 375,005 students

Access to over 1 million titles for a fair monthly price.

Study more efficiently using our study tools.

Information

Publisher

Year

Print ISBN

eBook ISBN

Topic

Subtopic

Index

Cover Page
Half-Title Page
Title Page
Copyright Page
Contents
Preface
About the Editors
Contributors
Chapter 1 Evaluating the Polyphasic Approach in Cyanobacterial Taxonomy
Chapter 2 Estimation of Growth and Photosynthetic Pigments in Cyanobacteria
Chapter 3 Quantitative Estimation of Total Carbohydrate, Protein and Lipid Content in Cyanobacteria
Chapter 4 Isolation and Purification of Phycobiliproteins
Chapter 5 Methods for Estimation of Oxidative Stress Indices in Cyanobacteria
Chapter 6 Estimation of Glutamine Synthetase Enzyme
Chapter 7 Estimation of Nitrate Reductase Enzyme
Chapter 8 Estimation of Nitrogenase Enzyme Activity in Cyanobacteria
Chapter 9 Isolation and Purification of Cyanobacterial Toxins
Chapter 10 Isolation and Purification of Scytonemin
Chapter 11 Isolation and Purification of Mycosporine-Like Amino Acids
Chapter 12 Protein Estimation in Cyanobacterial Samples
Chapter 13 Proteomic Analysis of Cyanobacteria Employing Two-Dimensional Gel Electrophoresis
Chapter 14 LC-MS/MS–Based Proteomics Study of Cyanobacteria
Chapter 15 Monitoring Photosynthetic Performance in Cyanobacteria by a Modulated Fluorometer
Chapter 16 Reactive Oxygen Species Measurement in Cyanobacteria Using 2′,7′-Dichlorodihydrofluorescein Diacetate (DCFH-DA) Dye and Fluorescence Microscope or Flow Cytometer
Chapter 17 Flow Cytometry–Based Methods for Estimating DNA Content and Live-Dead Cells in Cyanobacteria
Chapter 18 Genomic DNA and RNA Extraction from Cyanobacteria
Chapter 19 Detection and Quantification of DNA Damage in Cyanobacteria by Immunodot Blot Assay
Chapter 20 Engineering Cyanobacteria for the Synthesis of Novel Products
Chapter 21 Sample Preparation and Visualization Protocol for Electron Microscopy–Based Analysis of Cyanobacteria
Chapter 22 Cyanobacterial Biofertilizer Production Technology
Chapter 23 Biomass Composition Measurement and Intracellular Flux Analyses for Insights into Cyanobacterial Metabolism
Chapter 24 In Silico Genome Assembly and Functional Annotation
Index

Frequently asked questions

Yes, you can cancel anytime from the Subscription tab in your account settings on the Perlego website. Your subscription will stay active until the end of your current billing period. Learn how to cancel your subscription

No, books cannot be downloaded as external files, such as PDFs, for use outside of Perlego. However, you can download books within the Perlego app for offline reading on mobile or tablet. Learn how to download books offline

Perlego offers two plans: Essential and Complete

Essential is ideal for learners and professionals who enjoy exploring a wide range of subjects. Access the Essential Library with 800,000+ trusted titles and best-sellers across business, personal growth, and the humanities. Includes unlimited reading time and Standard Read Aloud voice.
Complete: Perfect for advanced learners and researchers needing full, unrestricted access. Unlock 1.4M+ books across hundreds of subjects, including academic and specialized titles. The Complete Plan also includes advanced features like Premium Read Aloud and Research Assistant.

Both plans are available with monthly, semester, or annual billing cycles.

We are an online textbook subscription service, where you can get access to an entire online library for less than the price of a single book per month. With over 1 million books across 990+ topics, we’ve got you covered! Learn about our mission

Look out for the read-aloud symbol on your next book to see if you can listen to it. The read-aloud tool reads text aloud for you, highlighting the text as it is being read. You can pause it, speed it up and slow it down. Learn more about Read Aloud

Yes! You can use the Perlego app on both iOS and Android devices to read anytime, anywhere — even offline. Perfect for commutes or when you’re on the go.
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app

Yes, you can access Methods in Cyanobacterial Research by Shailendra Pratap Singh, Rajeshwar P. Sinha, Donat-P. Häder, Shailendra Pratap Singh,Rajeshwar P. Sinha,Donat-P. Häder in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biology. We have over one million books available in our catalogue for you to explore.

About this book

Trusted by 375,005 students

Information

Table of contents

Frequently asked questions