Concepts and Models for Drug Permeability Studies

Cell and Tissue based In Vitro Culture Models

Bruno Sarmento, Catarina Leite Pereira, José Das Neves

• Publisher: Woodhead Publishing
• Year: 2024
• Edition: 2
• eBook ISBN:
  9780443155116
• Topic: Medicina
• Subtopic: Industria farmaceutica, biotecnologica e sanitaria

Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, summarizes the most important developments in in vitro models for predicting the permeability of drugs.This book is structured around three different approaches, summarizing the most recent achievements regarding models comprising (i) immortalized cells with an intrinsic ability to grow as monolayers when seeded in permeable supports, (ii) primary cells isolated from living organisms and directly cultured as barrier monolayers, and (iii) tissue-based models constructed with cell lines and extracellular matrix that resembles the tridimensional structure of mucosae and other biological membranes, or animal/patient-derived tissues. Each model is covered in detail, including the protocol of generation and application for specific drugs/drug delivery systems. The equivalence between in vitro cell and tissue models and in vivo conditions is discussed, highlighting how each model may provisionally resemble different drug absorption route.Chapters included in the first edition were updated with relevant data published in recent years, while four new chapters were included to reflect new emerging directions and trends in drug permeability models. Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, is a critical reference for drug discovery and drug formulation scientists interested in delivery systems intended for the administration of drugs through mucosal routes and other important tissue barriers (e.g. the BBB). Researchers studying mucosal biology can use this book to familiarize themselves and exploit the synergic effect of mucosal delivery systems and biomolecules. - Summarizes the current advances in the use of permeability models in drug transport - Covers the most important buccal, gastric, intestinal, pulmonary, nasal, vaginal, ocular, renal, skin, and blood–brain barrier in vitro models. Includes case studies to facilitate understanding of various concepts in computer-aided applications - Updates in the second edition include organ-on-chip devices, 3D advanced models (multiple layered tissues, organoids, etc.), and multicompartmentalized tissue models

Table of contents

• Concepts and Models for Drug Permeability Studies
• Chapter 1 Introduction
• Chapter 2 Importance and applications of cell- and tissue-based in vitro models for drug permeability screening in early stages of drug development
• Chapter 3.1 Cell-based in vitro models for buccal permeability studies
• Chapter 3.2 Cell-based in vitro models for gastric permeability studies
• Chapter 3.3 Cell-based in vitro models for intestinal permeability studies
• Chapter 3.4 Cell-based in vitro models for nasal permeability studies
• Chapter 3.5 Cell-based in vitro models for pulmonary permeability studies
• Chapter 3.6 Cell-based in vitro models for vaginal permeability studies
• Chapter 3.7 Cell-based in vitro models for transcorneal permeability studies
• Chapter 3.8 Cell-based in vitro models for dermal permeability studies
• Chapter 3.9 Tissue-based in vitro and ex vivo models for blood–brain barrier permeability studies
• Chapter 3.10 Cell-based in vitro models for placental barrier permeability studies
• Chapter 4.1 Tissue-based in-vitro models for buccal permeability studies
• Chapter 4.2 Tissue-based in vitro and ex vivo models for intestinal permeability studies
• Chapter 4.3 Tissue-based in vitro and ex vivo models for nasal permeability studies
• Chapter 4.4 Tissue-based in vitro and ex vivo models for pulmonary permeability studies
• Chapter 4.5 Tissue-based models for vaginal permeability studies
• Chapter 4.6 Tissue-based models for ocular permeability studies
• Chapter 4.7 Tissue-based in vitro and ex vivo models for dermal permeability studies
• Chapter 4.8 Tissue-based in vitro and ex vivo models for blood–brain barrier permeability studies
• Chapter 4.9 Tissue-based ex vivo models for placental barrier permeability studies
• Chapter 5.1 Organ-on-chip models for intestinal permeability studies
• Chapter 5.2 Organ-on-chip models for pulmonary permeability studies
• Chapter 5.3 Organ-on-chip models for blood–brain barrier permeability studies
• Chapter 5.4 Organ-on-chip models for other mucosal permeability studies
• Chapter 6.1 Multicompartmental dynamic models for permeability studies
• Chapter 6.2 Organoid-based models for permeability studies
• Chapter 7 Correlation between cell- and tissue-based in vitro models for drug permeability screening with in vivo situation: modeling and functional extrapolation
• Index