Mineralizing Vesicles

From Biochemical and Biophysical Properties to Their Roles in Physiology and Disease

Massimo Bottini, Ana Paula Ramos

• Publisher: Academic Press
• Year: 2023
• Print ISBN: 9780323991582
• eBook ISBN: 9780323991599
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

Mineralizing Vesicles: From Biochemical and Biophysical Properties to Their Roles in Physiology and Disease presents the state-of-the art in the properties of mineralizing EVs and their potential clinical applications. The first chapter presents foundational biochemical and biological aspects of EVs. Next, the title coves the role of EVs in bone ossification and in cardiovascular and cartilage-related diseases. Considering the unique ability of this class of EVs to form apatite minerals assigned to their special biochemical machinery, three chapters of the book then focus on the enzymes catalysing the inorganic phosphate and calcium turn-over and the dynamic properties of the vesicles' peripheral proteins. Chapters describe the role of inorganic phosphate and calcium ions and of autophagy on the biogenesis and function of mineralizing EVs. Recent studies show that the lumen of mineralizing EVs is partially filled with miRNA, and a chapter therefore considers research on the possible function of these vesicles as signalosomes. The final five chapters of the book describe practical aspects of working with mineralizing EVs, including their purification, proteomic and biophysical analyses, the use of biomimetic models and mineralizing EVs in regenerative medicine. This title presents, for the first time, a comprehensive account of mineralizing EVs and their potential clinical applications. It will be invaluable to researchers in the field. • Covers all aspects of mineralizing EVs, from their composition to their function in physiological   and pathological processes, and their clinical potential• Presents mineralizing EVs systematically using clear accessible language• Describes practical aspects of working with mineralizing EVs• Integrates an account of the biochemistry and biophysics of vesicles with their functions• Takes a multidisciplinary perspective, offering an exhaustive and cutting-edge account of mineralizing EVs and human health

Table of contents

• Mineralizing Vesicles
• Cover
• Title Page
• Copyright
• Table of Contents
• Contributors
• Biographies
• Preface
• Chapter 1 General aspects of extracellular vesicles in bone formation and remodeling
• Chapter 2 Physiological biomineralization. The properties and role of matrix vesicles in skeletal and dental calcifications
• Chapter 3 Pathological biomineralization. Part I: Mineralizing extracellular vesicles in cardiovascular diseases
• Chapter 4 Pathologic biomineralization: part II: mineralizing extracellular vesicles in osteoarthritis
• Chapter 5 The biochemistry of mineralizing extracellular vesicles. Part I: The role of phosphatases
• Chapter 6 The biochemistry of mineralizing extracellular vesicles. Part II: Annexins
• Chapter 7 Calcium and phosphate and their role in matrix vesicles: A biological view
• Chapter 8 Autophagy in bone metabolism and its possible role on the function of mineralizing extracellular vesicles
• Chapter 9 The roles of mineralizing extracellular vesicles in cell–cell communication
• Chapter 10 Working with mineralizing extracellular vesicles. Part I: Purification techniques
• Chapter 11 Working with mineralizing extracellular vesicles. Part II: Proteomic profiling
• Chapter 12 Working with mineralizing extracellular vesicles. Part III: The nucleational core
• Chapter 13 Working with mineralizing extracellular vesicles. Part IV: Biomimetic models
• Chapter 14 Working with mineralizing extracellular vesicles. Part V: Use of Mineralizing extracellular vesicles in bone regeneration
• Index