Subcellular Components
eBook - PDF

Subcellular Components

Preparation and Fractionation

  1. 332 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Subcellular Components

Preparation and Fractionation

About this book

Subcellular Components: Preparation and Fractionation talks about cells and particles' components, including their preparation and fractionation. The book includes theories and answers to questions that are relevant to the study. The first chapter of the book details various facts about homogenization of mammalian cells. This chapter presents the results of studies on solid tissues and single-cell suspensions; the author then offers his conclusion of the study. The next two chapters highlight the methods on isolating nuclei, including the guides for standard assessment and the procedure of isolation, along with analysis of nuclei biochemical properties. The main topics in Chapter 4 are mitochondria from animal tissues and yeasts; this chapter also discusses the preparation for a rat-liver, blowfly flight-muscle, yeast, and brain mitochondria. The chapter that follows widely talks about lysosomes, including its historical background, centrifugal method, and related topics. In the next several chapters, the topics covered include purification, isolation, preparation, and separation of cells including plasma-membrane, polysomes, ribosomes, microsomes, and microvilli. The book serves as a great reference for undergraduates and postgraduates in the field, as it contains a thorough discussion of various relevant studies.

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Yes, you can access Subcellular Components by G. D. Birnie in PDF and/or ePUB format, as well as other popular books in Technology & Engineering & Biochemistry. We have over one million books available in our catalogue for you to explore.

Table of contents

  1. Front Cover
  2. Subcellular Components: Preparation and Fractionation
  3. Copyright Page
  4. Table of Contents
  5. CHAPTER 1. PRESSURE HOMOGENISATION OF MAMMALIAN CELLS
  6. CHAPTER 2. SOME METHODS FOR ISOLATING NUCLEI
  7. CHAPTER 3. THE BIOCHEMICAL PROPERTIES OF NUCLEI FRACTIONATED BY ZONAL CENTRIFUGATION
  8. CHAPTER 4. PREPARATION OF MITOCHONDRIA FROM ANIMAL TISSUES AND YEASTS
  9. CHAPTER 5. PREPARATION OF LYSOSOME-RICH FRACTIONS WITH OR WITHOUT PEROXISOMES
  10. CHAPTER 6. PURIFICATION OF PLASMA-MEMBRANE FRAGMENTS
  11. CHAPTER 7. THE ISOLATION OF BRUSH BORDERS (MICROVILLI) FROM THE EPITHELIAL CELLS OF MAMMALIAN INTESTINE
  12. CHAPTER 8. PREPARATION AND PROPERTIES OF MICROSOMAL AND SUBMICROSOMAL FRACTIONS FROM SECRETORY AND NON-SECRETORY TISSUES
  13. CHAPTER 9. ISOLATION OF ANIMAL POLYSOMES AND RIBOSOMES
  14. CHAPTER 10. SEPARATION OF POLYSOMES, RIBOSOMES AND RIBOSOMAL SUBUNITS IN ZONAL ROTORS
  15. CHAPTER 11. ISOLATION AND FRACTIONATION OF RNA
  16. CHAPTER 12. ISOPYCNIC CENTRIFUGATION OF DNA METHODS AND APPLICATIONS
  17. INDEX