Mitochondrial Dysfunction

Methods in Toxicology, Vol. 2

Lawrence H. Lash, Dean P. Jones

• Publisher: Academic Press
• Year: 2013
• Print ISBN: 9780124612051
• eBook ISBN: 9781483218618
• Topic: Biological Sciences
• Subtopic: Toxicology
• Index: Biological Sciences

Methods in Toxicology, Volume 2: Mitochondrial Dysfunction provides a source of methods, techniques, and experimental approaches for studying the role of abnormal mitochondrial function in cell injury. The book discusses the methods for the preparation and basic functional assessment of mitochondria from liver, kidney, muscle, and brain; the methods for assessing mitochondrial dysfunction in vivo and in intact organs; and the structural aspects of mitochondrial dysfunction are addressed. The text also describes chemical detoxification and metabolism as well as specific metabolic reactions that are especially important targets or indicators of damage. The methods for measurement of alterations in fatty acid and phospholipid metabolism and for the analysis and manipulation of oxidative injury and antioxidant systems are also considered. The book further tackles additional methods on mitochondrial energetics and transport processes; approaches for assessing impaired function of mitochondria; and genetic and developmental aspects of mitochondrial disease and toxicology. The text also looks into mitochondrial DNA synthesis, covalent binding to mitochondrial DNA, DNA repair, and mitochondrial dysfunction in the context of developing individuals and cellular differentiation. Microbiologists, toxicologists, biochemists, and molecular pharmacologists will find the book invaluable.

Table of contents

• Front Cover
• Mitochondrial Dysfunction
• Copyright Page
• Dedication
• Table of Contents
• Contributors
• Preface
• Introduction: Criteria for Assessing Normal and Abnormal Mitochondrial Function
• Chapter 1. Mitochondrial Isolation from Liver and Kidney: Strategy, Techniques, and Criteria for Purity
• Chapter 2. Mitochondrial Isolation from Brain: Strategy, Techniques, and Criteria for Purity
• Chapter 3. Intact Rat Brain Mitochondria from a Single Animal: Preparation and Properties
• Chapter 4. Study of Skeletal Muscle Mitochondrial Dysfunction
• Chapter 5. Small-Scale Preparation of Skeletal Muscle Mitochondria and Its Application in the Study of Human Disease
• Chapter 6. Metabolie Control Analysis as a Method to Assess Mitochondrial Dysfunction
• Chapter 7. Commentary: Methods for in Vivo Assessment of Mitochondrial Function
• Chapter 8. Noninvasive Assessment of Mitochondrial Function by Breath Analysis Using Ketoisocaproic Acid
• Chapter 9. Absorption Spectroscopy for Assessment of Mitochondrial Function in Vivo
• Chapter 10. Analysis of Mitochondrial Function by Carbon-13 Nuclear Magnetic Resonance Spectroscopy in Intact Tissues
• Chapter 11. Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy in the Study of Mitochondrial Metabolism
• Chapter 12. Mitochondrial Dysftinctíon in Ischemic Organs
• Chapter 13. Screening for Mitochondrial Cytopathy: The Subanaerobic Threshold Exercise Test
• Chapter 14. Morphometry of Mitochondria: Size, Internal Structure, Subcellular Distribution, and Three-Dimensional Reconstruction
• Chapter 15. Megamitochondria
• Chapter 16. Redox Status and Mitochondrial Inner Membrane Permeability
• Chapter 17. Assessment of Mitochondrial Glutathione as a Measure of Cell Injury
• Chapter 18. Experimental Manipulation of Mitochondrial Glutathione Concentrations
• Chapter 19. Determination of Flux, Activity, and Activity State of Mitochondrial α-Keto Acid Dehydrogenase Complexes
• Chapter 20. Regulation of Oxygen Uptake in the Liver Lobule by Oxygen Tension
• Chapter 21. Generation of Reactive Oxygen Metabolites and Oxidative Damage in Mitochondria: Role of Calcium
• Chapter 22. Fatty Acid Metabolism and Reye's Syndrome
• Chapter 23. Antioxidative Function of Vitamin Ε and Ubiquinols
• Chapter 24. Mitochondrial NADPH
• Chapter 25. Long-Chain Acyl-CoA Metabolism by Mitochondrial Carnitine Palmitoyltransferase: A Cell Model for Pathological Studies
• Chapter 26. Identification of Mitochondrial Dysfunction at Coupling Site I: Loss of Activity of NADH-Ubiquinone Oxidoreductase during Myocardial Ischemia
• Chapter 27. Identification of Mitochondrial Dysfunction at Coupling Site II
• Chapter 28. Cellular Calcium and Mitochondrial Dysfunction
• Chapter 29. Chronic Alcoholism and the Mitochondrial F0F1-ATP Synthase
• Chapter 30. Mitochondrial Pyrophosphate Metabolism in Health and Disease
• Chapter 31. Toxic Effects of Calcium on Mitochondria
• Chapter 32. Calcium and the Regulation of Intramitochondrial Dehydrogenases
• Chapter 33. Use of Fluorescent Probes to Monitor Mitochondrial Membrane Potential in Isolated Mitochondria, Cell Suspensions, and Cultured Cells
• Chapter 34. Commentary: Genetic Approaches to Mitochondrial DNA Diseases of Oxidative Phosphorylation
• Chapter 35. Developmental Aspects of Mitochondrial Dysfiinction
• Chapter 36. Mitochondrial DNA Synthesis
• Chapter 37. Covalent Binding of Reactive Intermediates of Xenobiotics to Mitochondrial DNA
• Chapter 38. Mitochondrial DNA Repair and Cell Injury
• Index