Methods of Cell Separation
eBook - PDF

Methods of Cell Separation

  1. 287 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Methods of Cell Separation

About this book

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method.Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes.An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

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Table of contents

  1. Front Cover
  2. Methods of Cell Separation
  3. Copyright Page
  4. Contents
  5. Abbreviations
  6. Chapter 1. Introduction
  7. Chapter 2. Methods of cell counting and assaying cell viability
  8. Chapter 3. Centrifugation
  9. Chapter 4. Unit gravity sedimentation
  10. Chapter 5. Centrifugal elutriation
  11. Chapter 6. Aqueous two-phase partition
  12. Chapter 7. Electrophoresis
  13. Specific surface methods – an introduction to Chapters 8–13
  14. Chapter 8. Affinity chromatography
  15. Chapter 9. Affinity separation on fibres and surfaces
  16. Chapter 10. Affinity partition
  17. Chapter 11. Flow sorting
  18. Chapter 12. Magnetic cell sorting
  19. Chapter 13. Separation of cells in different phases of the cell cycle
  20. Appendix
  21. References
  22. General references for further reading
  23. Subject index

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Yes, you can access Methods of Cell Separation by P.T. Sharpe in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Physiology. We have over one million books available in our catalogue for you to explore.