Protocols of Actinomycetes

Microbiology to Gene editing

L Karthik

• Publisher: CRC Press
• Year: 2024
• eBook ISBN: 9781040225721
• Edition: 0
• Topic: Medicine
• Subtopic: Biology

Many of the natural products have been isolated and characterized from the actinobacteria, for example polyketides, phenazines, peptides, indolocarbarbazoles, and sterols. To explore new antibiotics from actinobacteria, several bioinformatics and synthetic biology tools were developed. This book covers basics to recent protocols for drug discovery from actinobacteria.

Features:

• Discusses the benefits of production of antibiotics and enzymes from actinomycetes in a large scale
• Covers the synthetic biology approach
• Describes the strain improvement of actinobacteria
• Gives information on basic isolation of actinobacteria and modern techniques
• Covers the applications and metabolic engineering strategies of actinomycetes

This book will be helpful for the pharmaceutical industries and researchers to develop new antibiotics from actinobacteria and can be used in support of future research in drug discovery.

Table of contents

• Cover
• Half Title
• Title Page
• Copyright Page
• Dedication
• Table of Contents
• Foreword
• Preface
• About the Editor
• Contributors
• Chapter 1 Isolation of Actinomycetota by Different Methods
• Chapter 2 Recent Traditional Methods for Isolating Diverse Actinobacteria
• Chapter 3 Isolation of Actinobacteria from Caves and Limestone Habitats
• Chapter 4 Isolation of Endophytic Actinobacteria from Different Sources
• Chapter 5 Sample Treatment and Protocols for the Cultivation of Marine Actinomycetes
• Chapter 6 A Protocol for Screening, Cultivation, and Molecular Identification of Rare and Slow-Growing Actinomycetes with Biosynthetic Gene Clusters
• Chapter 7 Isolation and Screening of Enzymes from Actinobacteria
• Chapter 8 RSM-Based Media Optimization for Enzyme Production by Streptomyces
• Chapter 9 Protocols for the Screening, Extraction, and Purification of the Hydrolytic Enzymes from Marine Actinomycetes
• Chapter 10 Marine Actinomycetes’ Hydrolytic Enzymes: A Structural Characterization
• Chapter 11 Isolation of Beta-Glucanase Encoding Genes from Actinobacterial Genus Streptomyces spp. and its Overexpression in Escherichia coli Heterologous System
• Chapter 12 Isolation and Screening of Enzyme Inhibitors from Marine Actinobacteria
• Chapter 13 Screening of Amylase and Lipase Enzyme Inhibitors from Actinomycetota
• Chapter 14 Screening and Characterization of Carotenoid Pigment from the Actinobacteria
• Chapter 15 Screening of Biosurfactant Activity of Actinobacteria
• Chapter 16 Traditional Screening of Actinomycetes for Antibacterial, Antifungal, and Antiviral Properties
• Chapter 17 Isolation and Screening of Actinobacteria with Antibacterial Activity and Its Production through Shake Flask Culture Method118
• Chapter 18 Screening of Antimycobacterial Property of Actinobacteria
• Chapter 19 Screening Actinomycetes for Novel Antimycobacterial and Anticancer Molecules
• Chapter 20 In Vitro Antioxidant Protocols for Actinobacteria
• Chapter 21 Protocol for Antileishmanial Activity Using Marine Actinobacteria
• Chapter 22 Shining a Light on the Potent Antimalarial Activity of Actinomycetes Extract: A Comprehensive In Vitro and In Vivo Evaluation
• Chapter 23 Screening of Marine Actinobacteria for Postprandial Antidiabetic Activity In-Vitro and In-Vivo Methods161
• Chapter 24 Exploring the Larvicidal Activity of Actinomycetes Extracts
• Chapter 25 Evaluation of Actinobacteria’s Antibiofilm Properties Using Qualitative and Quantitative Methods170
• Chapter 26 Microplastic Degradation by Actinobacteria
• Chapter 27 Antibacterial and Antibiofilm Activity of Biosynthesized Nanoparticles from Actinomycetes: An Alternative, Environmental-Free, Sustainable Approach
• Chapter 28 Extraction and Purification of Rapamycin from Streptomyces hygroscopicus
• Chapter 29 Isolation and Identification of Squalene from the Ethyl Acetate Extract of Streptomyces sp. VITGV156 (MCC 4965)
• Chapter 30 Docking and ADME Studies Protocol
• Chapter 31 Methods and Protocols for the Extraction of the Genomic DNA, Gene Cloning, and Heterologous Expression from the Actinomycetes of Extreme Habitats
• Chapter 32 Activation of Silent Genes in Actinomycetes by Different Methods
• Chapter 33 Multimodal Spectroscopy-Based Proteomics and Metabolomics Approach to Explore Actinomycetes221
• Chapter 34 Isolation of DNA and RNA from Actinobacteria
• Chapter 35 Extraction of DNA from Actinomycetota and Amplification of the 16S rRNA Gene
• Chapter 36 Optimization of Colony Polymerase Chain Reaction for the Identification of Actinobacteria by Sequencing of the 16S rDNA245
• Chapter 37 Identification of Actinomycetes by Polyphasic Taxonomy
• Chapter 38 Phylogenetic Analysis of Actinomycetes
• Chapter 39 Genetic Profiling of Actinobacterial Genomes through Polymerase Chain Reaction-based Techniques
• Chapter 40 In Silico Analysis of Phylogenetic Relationship and Non-synonymous Polymorphisms in Actinobacteria
• Chapter 41 Isolation of Metagenomic DNA and Data Analysis
• Chapter 42 Practical Methodology of Illumina Sequencing Investigating Actinobacteria Genomes
• Chapter 43 Digital DNA-DNA Hybridization for the Identification of Actinomycetota Species
• Chapter 44 antiSMASH: Genome Mining and Genome-Wide Analysis of Gene Clusters in Actinopolyspora Species
• Chapter 45 Transcriptome Analysis of Actinomycetes RNA Sequencing Data: A Bioinformatics Protocol for Beginners
• Chapter 46 Single-Cell RNA Sequencing of the Actinomycetes
• Chapter 47 Preparation of Streptomyces RNA for Transcriptomics
• Chapter 48 A Protocol for CRISPR-Cas9-Mediated Knockout Construction in Actinobacteria
• Chapter 49 Design of gRNA for DNA-Directed RNA Polymerase Beta Subunit (rpoB) Gene in Actinomycetes
• Chapter 50 Acute and Chronic Toxicity of Bioactive Compounds from Actinobacteria
• Chapter 51 Production, Extraction, Purification, and Characterization of Bioactive Derivatives of Phthalate Ester from Marine Streptomyces sp. VITJS4
• Index