Protocols in Biochemistry and Clinical Biochemistry
eBook - ePub

Protocols in Biochemistry and Clinical Biochemistry

  1. 320 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Protocols in Biochemistry and Clinical Biochemistry

About this book

Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results. - Includes full listings and discussions of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting across 100 protocols - Features clear, step-by-step instruction with color diagrams and images, followed by worked examples of putting lab techniques into action - Empowers researchers and clinicians to reproduce research and clinical methods and ensure test accuracy

Frequently asked questions

Yes, you can cancel anytime from the Subscription tab in your account settings on the Perlego website. Your subscription will stay active until the end of your current billing period. Learn how to cancel your subscription.
At the moment all of our mobile-responsive ePub books are available to download via the app. Most of our PDFs are also available to download and we're working on making the final remaining ones downloadable now. Learn more here.
Perlego offers two plans: Essential and Complete
  • Essential is ideal for learners and professionals who enjoy exploring a wide range of subjects. Access the Essential Library with 800,000+ trusted titles and best-sellers across business, personal growth, and the humanities. Includes unlimited reading time and Standard Read Aloud voice.
  • Complete: Perfect for advanced learners and researchers needing full, unrestricted access. Unlock 1.4M+ books across hundreds of subjects, including academic and specialized titles. The Complete Plan also includes advanced features like Premium Read Aloud and Research Assistant.
Both plans are available with monthly, semester, or annual billing cycles.
We are an online textbook subscription service, where you can get access to an entire online library for less than the price of a single book per month. With over 1 million books across 1000+ topics, we’ve got you covered! Learn more here.
Look out for the read-aloud symbol on your next book to see if you can listen to it. The read-aloud tool reads text aloud for you, highlighting the text as it is being read. You can pause it, speed it up and slow it down. Learn more here.
Yes! You can use the Perlego app on both iOS or Android devices to read anytime, anywhere — even offline. Perfect for commutes or when you’re on the go.
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app.
Yes, you can access Protocols in Biochemistry and Clinical Biochemistry by Buddhi Prakash Jain,Shweta Pandey,Shyamal K Goswami in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biochemistry. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2024
eBook ISBN
9780443139468
Edition
2
Topic
Biological Sciences
Subtopic
Biochemistry
Index
Biological Sciences

Table of contents

  1. Cover
  2. Front Matter
  3. Table of Contents
  4. Copyright
  5. Author Biography
  6. Techniques
  7. List of Illustrations
  8. List of Tables
  9. Chapter 1 : Preparation of Solutions
  10. Chapter 2 : Acids and Bases
  11. Chapter 3 : Determination of the Presence of Carbohydrates in the Given Sample by Molisch’s Test
  12. Chapter 4 : Determination of the Presence of Reducing Carbohydrates in the Given Sample by Picric Acid Test
  13. Chapter 5 : Determination of the Presence of Reducing Carbohydrates in the Given Sample by Fehling’s Test
  14. Chapter 6 : Determination of the Presence of Reducing Carbohydrates in the Given Sample by Benedict’s Test
  15. Chapter 7 : Determination of the Presence of Reducing Carbohydrates in the Given Sample by Tommer’s Test
  16. Chapter 8 : Determination of the Presence of Reducing Carbohydrates in the Given Sample by Nylander’s Test
  17. Chapter 9 : Detection of the Presence of Monosaccharides and Reducing Disaccharides by Barfoed’s Test
  18. Chapter 10 : Differentiation Between Aldose and Ketose Sugars by Seliwanoff’s Test
  19. Chapter 11 : Determination of Pentose Sugar by Bial’s Test
  20. Chapter 12 : Determination of the Presence of Galactose in the Test Sample by the Mucic Acid Test
  21. Chapter 13 : Differentiation Between the Presence of Ketohexose and Aldohexose in the Test Sample by Foulger’s Test
  22. Chapter 14 : Determination of Reducing Carbohydrates by Fearon’s Methylamine Test
  23. Chapter 15 : Confirmation of the Presence of Reducing Carbohydrates in the Given Sample by Osazone Formation Test
  24. Chapter 16 : Determination of the Presence of Polysaccharides in the Given Sample by Iodine Test
  25. Chapter 17 : Quantitative Estimation of Carbohydrates in the Given Sample by Anthrone Test
  26. Chapter 18 : Quantitative Estimation of Reducing Carbohydrates in the Given Sample by 3,5-Dinitro Salicylic Acid Test
  27. Chapter 19 : Extraction and Estimation of Glycogen from the Liver and Muscle of the Well-Fed and Starved Rat
  28. Chapter 20 : Separation and Identification of Sugars Present in Fruit Juices Using Thin-Layer Chromatography (TLC)
  29. Chapter 21 : Extraction and Analysis of Soluble Carbohydrates From Plants
  30. Chapter 22 : Solubility Test for Lipids
  31. Chapter 23 : Emulsion Test for Lipids
  32. Chapter 24 : Determination of the Degree of Unsaturation of Fatty Acids
  33. Chapter 25 : Determination of the Saponification Value of the Given Fat/Oil Sample
  34. Chapter 26 : Determination of the Fatty Acid Value of Fat
  35. Chapter 27 : Acrolein Test for the Presence of Glycerol
  36. Chapter 28 : Liebermann-Burchard Test for Detection of Cholesterol
  37. Chapter 29 : Extraction of Lipid From Leaves (Bligh and Dyer’s Method and Single Extraction Method)
  38. Chapter 30 : Extraction of Lipid From Egg Yolk and Estimation of Phosphorous Content in It
  39. Chapter 31 : Extraction of Lipid From Tissues (Folch Method)
  40. Chapter 32 : Separation and Identification of Lipids by Thin-Layer Chromatography
  41. Chapter 33 : Lipid Peroxidation Measurement From Biological Samples by Thiobarbituric Acid (TBA) Assay
  42. Chapter 34 : Isolation of Proteins From Animal Cells/Tissue [Using Radioimmunoprecipitation Assay (RIPA) Buffer]
  43. Chapter 35 : Qualitative Test for Proteins and Amino Acids
  44. Chapter 36 : Estimation of Proteins by the Biuret Method
  45. Chapter 37 : Estimation of Proteins by the Bradford Method
  46. Chapter 38 : Estimation of Proteins by Bicinchoninic Acid (BCA) Method
  47. Chapter 39 : Protein Estimation by Folin-Ciocalteau (Lowry) Method
  48. Chapter 40 : Estimation of Proteins by UV Spectrophotometric Method
  49. Chapter 41 : Protein Separation by SDS-PAGE
  50. Chapter 42 : Separation of Amino Acids by Paper Chromatography
  51. Chapter 43 : Estimation of Calcium in Water or Milk by EDTA Titrimetric Method
  52. Chapter 44 : Estimation of the Calcium in Serum by O-Cresolpthalein Complexon Method (Colorimetric)
  53. Chapter 45 : Estimation of the Inorganic Phosphorus by Fiske and Subbarow Method
  54. Chapter 46 : Estimation of Iron by Thiocyanate Colorimetric Method
  55. Chapter 47 : Estimation of Chloride by Titration (Mohr Argentometric) Method
  56. Chapter 48 : Estimation of Vitamin A by Trichloroacetic Acid (Modified Carr-Price Method)
  57. Chapter 49 : Estimation of the Tocopherol by Baker and Frank Method
  58. Chapter 50 : Estimation of the Thiamine (Vitamin B1) by Thiochrome Technique (Fluorimetric)
  59. Chapter 51 : Estimation of Vitamin C (Ascorbic Acid) by Indophenol Method in a Given Solution (Titrimetric Method)
  60. Chapter 52 : Estimation of Vitamin C by 2,4-Dinitrophenylhydrazine Method
  61. Introduction
  62. Chapter 53 : Study of the Effect of pH and Temperature on the Action of Salivary Amylase
  63. Chapter 54 : Determination of Activity of Amylase Enzyme (Crude) Extracted From Sweet Potato
  64. Chapter 55 : Determination of the Specific Activity of Amylase Enzyme
  65. Chapter 56 : Determination of Vmax and Km of Amylase Enzyme
  66. Chapter 57 : Determination of the Amount of DNA Present in the Solution by Diphenylamine (DPA) Method
  67. Chapter 58 : Determination of RNA by Orcinol Method
  68. Chapter 59 : Isolation of Plasmid DNA Using Alkaline Lysis Method
  69. Chapter 60 : Preparation of Plasmid DNA Using Boiling Lysis Method
  70. Chapter 61 : Plasmid DNA Purification Using Polyethylene Glycol
  71. Chapter 62 : Isolation of Genomic DNA of Unknown Bacterial Strain
  72. Chapter 63 : Isolation of DNA From Plant Tissue
  73. Chapter 64 : Isolation of DNA From Eukaryotic Cells Using Proteinase K and Phenol
  74. Chapter 65 : Isolation of DNA From Eukaryotic Cells
  75. Chapter 66 : Separation of DNA by Agarose Gel Electrophoresis
  76. Chapter 67 : Southern Blotting
  77. Chapter 68 : Isolation of RNA From Animal Cells
  78. Chapter 69 : Northern Blotting
  79. Chapter 70 : Determination of Serum Urea [Diacetyl Monoxime (DAM) Method]
  80. Chapter 71 : Estimation of Uric Acid in Serum Sample (Uricase Method)
  81. Chapter 72 : Estimation of Creatinine in Serum Samples (Jaffe’s Method)
  82. Chapter 73 : Determination of Serum Glutamate Pyruvate Transaminase (SGPT) Activity
  83. Chapter 74 : Determination of Serum Glutamate Oxaloacetate Transaminase (SGOT) Activity
  84. Chapter 75 : Colorimetric Estimation of Alkaline Phosphatase (ALP) From Serum
  85. Chapter 76 : Estimation of Serum Albumin by Dye-Binding Method
  86. Chapter 77 : Estimation of Blood Glucose by Glucose Oxidase Method
  87. Chapter 78 : Estimation of Glucose in Urine by Benedict's Method
  88. Chapter 79 : Estimation of Percentage of Glucose (Reducing Sugars) in Urine (by Benedict Titration Method)
  89. Chapter 80 : Oral Glucose Tolerance Test
  90. Chapter 81 : Estimation of Total Cholesterol in Serum (Watson Method)
  91. Chapter 82 : Estimation of Total Cholesterol in Serum (Enzymatic Method)
  92. Chapter 83 : Estimation of Triglycerides in Serum (Enzymatic Method)
  93. Chapter 84 : Estimation of Hemoglobin in the Blood (Sahli’s Method)
  94. Chapter 85 : Estimation of Serum Amylase (Kinetic Colorimetric Method)
  95. Chapter 86 : Estimation of Catalase Activity in Biological Samples
  96. Chapter 87 : Superoxide Dismutase (SOD) Assay (Marklund et al., 1974)
  97. Chapter 88 : Qualitative Analysis of Secondary Metabolite of Plants
  98. Chapter 89 : Estimation of Ascorbic Acid in Plant Samples
  99. Chapter 90 : Quantitative Analysis of Secondary Metabolite of Plants
  100. Chapter 91 : Estimation of Chlorophyll Content of Plant
  101. Chapter 92 : Determination of Starch
  102. GENERIC-TITLE
  103. Index
  104. A