Cleanroom Microbiology for the Non-Microbiologist
eBook - ePub

Cleanroom Microbiology for the Non-Microbiologist

  1. 216 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Cleanroom Microbiology for the Non-Microbiologist

About this book

Written for the professional who has an immediate need for the information but has little or no training in the subject, Cleanroom Microbiology for the Non-Microbiologist, Second Edition introduces principles of microbiology. It explains the consequences of microbiological contamination, what contamination is all about, how microorganisms grow, and

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Yes, you can access Cleanroom Microbiology for the Non-Microbiologist by David M. Carlberg in PDF and/or ePUB format, as well as other popular books in Medicine & Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
CRC Press
Year
2004
eBook ISBN
9781040190777
Topic
Medicine
Subtopic
Biology

Table of contents

  1. Cover
  2. Half Title
  3. Title Page
  4. Copyright Page
  5. Dedication
  6. Preface To The Second Edition
  7. Preface To The First Edition
  8. Acknowledgments
  9. The Author
  10. Table of Contents
  11. 1 The Scope of Microbiology
  12. I. Introduction
  13. II. Cells
  14. III. Binomial Nomenclature
  15. IV. Morphology
  16. V. Specific Microbial Groups
  17. A. Bacteria
  18. 1. The Gram Stain
  19. 2. External Features of Bacterial Cells
  20. 3. The Bacterial Interior
  21. 4. Bacterial Genetics
  22. B. Fungi
  23. 1. Molds
  24. 2. Yeasts
  25. C. Algae
  26. D. Protozoa
  27. E. Viruses
  28. 1. The Viral Infection Cycle
  29. 2. Retroviruses
  30. 3. Oncoviruses
  31. 4. Viruses and Gene Therapy
  32. F. Subviral Particles
  33. 1. Viroids
  34. 2. Satellite RNA
  35. 3. Prions
  36. VI. Taxonomy of Microorganisms
  37. VII. Microscopy
  38. A. Light Microscopy
  39. 1. Staining
  40. 2. Types of Light Microscopes
  41. B. Electron Microscopy
  42. VIII. Summary
  43. 2 Growth of Microorganisms
  44. I. Introduction
  45. A. Scientific Notation
  46. II. Growth of Bacteria
  47. A. Nutritional Requirements
  48. B. Environmental Requirements
  49. 1. Temperature
  50. 2. Atmosphere
  51. 3. pH
  52. C. Binary Fission and the Bacterial Growth Curve
  53. 1. Microbe Math
  54. 2. Characteristics of Bacterial Populations
  55. 3. The Growth Curve
  56. D. Continuous Growth
  57. E. Determining Bacterial Populations in Cultures
  58. 1. The Viable Plate Count
  59. 2. Filtration Methods
  60. 3. Total Cell Counts
  61. 4. Instrumental Methods
  62. F. Microorganisms as Tools
  63. III. Growing Other Microorganisms: Fungi, Protozoa, Algae,and Viruses
  64. A. Fungi
  65. B. Protozoa and Algae
  66. C. Viruses
  67. 1. Counting Viruses
  68. IV. Aseptic Technique
  69. V. Safety Issues
  70. VI. Summary
  71. 3 Controlling Growth and Activities of Microorganisms
  72. I. Introduction
  73. II. Definitions
  74. III. Physical Methods of Control
  75. A. The Kinetics of Microbial Death
  76. B. Heat
  77. 1. Moist Heat
  78. 2. Dry Heat
  79. 3. Sterilizing Large Loads
  80. C. Low Temperatures
  81. D. Radiation
  82. 1. Ionizing Radiation
  83. 2. Nonionizing Radiation
  84. E. Filtration
  85. 1. Depth Filters
  86. 2. Membrane Filters
  87. 3. Mechanisms of Filtration
  88. 4. Validation of Filtration Sterilization
  89. IV. Ensuring Sterility
  90. A. Overkill Method
  91. B. Bioburden Method
  92. C. Validation of Sterilization Processes
  93. D. Maintaining Sterility
  94. V. Chemical Methods of Control
  95. A. Definitions
  96. B. Factors Influencing Antimicrobial Chemical Agents
  97. C. Disinfectants
  98. 1. Characteristics of Specific Agents
  99. 2. Selection of Disinfectants
  100. 3. Validation of Disinfectants
  101. 4. Rotation of Disinfectants
  102. D. Sterilants
  103. 1. Characteristics of Specific Agents
  104. E. Antibiotics
  105. VI. Control of Microorganisms in High-Purity Water Systems
  106. A. The Need for Pure Water
  107. B. Water Purification Methods
  108. C. Biofilms
  109. VII. Summary
  110. 4 Cleanroom Facilities and Personnel Controls
  111. I. Introduction
  112. II. Certified Cleanrooms
  113. III. Bioclean Facilities — Viable vs. Nonviable Particles
  114. IV. Clean Facilities — General Considerations
  115. V. Unidirectional Air Flow
  116. VI. HEPA and ULPA Filters
  117. VII. Aseptic Fill Areas
  118. VIII. Barriers, Clean Zones, and Isolators
  119. A. Biological Safety Cabinets
  120. 1. Class I BSCs
  121. 2. Class II BSCs
  122. 3. Class III BSCs
  123. 4. Proper BSC Operation
  124. 5. BSC Certification
  125. B. Isolators
  126. C. Clean Benches
  127. IX. Biosafety Levels
  128. A. BSL4 Facilities
  129. B. Positive-Pressure Personnel Suits
  130. X. Cleanroom Garments
  131. A. Correct Gowning Procedures
  132. B. Sterile Gowning
  133. XI. Personnel Practices and Training
  134. XII. Personal Hygiene
  135. XIII. General Personnel Practices
  136. XIV. Summary
  137. 5 Detection and Enumeration of Microorganisms in the Cleanroom
  138. I. Introduction
  139. II. Overview of Microbiological Environmental Monitoring
  140. A. Validation
  141. B. Monitoring Techniques.
  142. 1. General Considerations
  143. 2. Sampling Sites
  144. 3. Frequency of Sampling
  145. 4. Transporting Microbiological Samples
  146. III. Monitoring of Air
  147. A. Air Samplers
  148. 1. The Andersen Sampler
  149. 2. The Slit Sampler
  150. 3. Portable Samplers
  151. 4. Liquid Impingers
  152. 5. Sampler Efficiencies
  153. IV. Fallout Methods
  154. V. Surface Monitoring
  155. VI. Touch Plates and Other Personnel Monitoring
  156. VII. Microbiological Assessment of Liquids
  157. VIII. Microbiological Assessment of Solids and Semisolids
  158. IX. Monitoring for Specific Microorganisms
  159. A. Bacteria
  160. 1. Bacterial Nutrition and Choice of Growth Media
  161. B. Fungi
  162. 1. Fungal Nutrition and Choice of Growth Media
  163. X. Rapid Methods of Identification
  164. XI. Testing for Bacteriostasis and Fungistasis
  165. XII. Detection of Biofilms
  166. XIII. Sterile Media Fill Tests
  167. XIV. Alert and Action Levels
  168. XV. The Sterility Suite
  169. XVI. Disposal of Cultures
  170. XVII. Summary
  171. References
  172. Index