Gene Biotechnology
eBook - ePub

Gene Biotechnology

  1. English
  2. ePUB (mobile friendly)
  3. Available on iOS & Android
eBook - ePub

Gene Biotechnology

About this book

Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the princ

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Yes, you can access Gene Biotechnology by William Wu,Helen H. Zhang,Michael J. Welsh,Peter B. Kaufman in PDF and/or ePUB format, as well as other popular books in Medicine & Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
CRC Press
Year
2016
eBook ISBN
9781040221921
Topic
Medicine
Subtopic
Biology

Table of contents

  1. Cover
  2. Title Page
  3. Copyright Page
  4. Table of Contents
  5. Preface
  6. Authors
  7. 1 Strategies for Novel Research Projects and/or Research Grant Funding
  8. 2 Rapid Isolation of Specific cDNAs orGenes by PCR
  9. 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries
  10. 4 Subcloning of Genes or DNA Fragments
  11. 5 Nonisotopic and Isotopic DNA or RNA Sequencing
  12. 6 Bioinformation Superhighway and Computer Databases of Nucleic Acids
  13. 7 Characterization of DNA or Genes by Southern Blot Hybridization
  14. 8 Gene Overexpression by Sense RNA inMammalian Systems
  15. 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
  16. 10 Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR
  17. 11 Analysis of Gene Expression at Proteomic Level via Western Blotting
  18. 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization
  19. 13 Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization
  20. 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs
  21. 15 Isolation and Characterization of Genes from Genomic DNA Libraries
  22. 16 Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression
  23. 17 Strategies for Gene Double Knockout
  24. 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells
  25. 19 Quantitative Analysis of Functional Genome by Real-Time RT-PCR
  26. 20 High-Throughput Analysis of Gene Expression by CuttingEdge Technology—DNA Microarrays (Gene Chips)
  27. 21 Construction and Screening of Human Antibody Libraries Using Phage Display Technology
  28. 22 Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology
  29. 23 Strategies for Gene Cloning, Expression, and Identification of Protein- Protein Interaction
  30. 24 Conditional Gene Knockout
  31. 25 How to Write a Research Manuscript for Publication in an English Journal
  32. 26 How to Protect Your Discovery and Invention
  33. 27 Determination of Transgene Copy Numbers and Practical Biocalculation