Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation

Shan S. Wong, David M. Jameson

• Publisher: CRC Press
• Year: 2011
• eBook ISBN:
  9781040202777
• Topic: Physical Sciences
• Subtopic: Biochemistry

Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross

Table of contents

• Cover
• Half Title
• Title Page
• Copyright Page
• Contents
• Preface
• Authors
• 1 Overview of Protein Conjugation
• 2 Review of Protein and Nucleic Acid Chemistry
• 3 Reagents Targeted to Specific Functional Groups
• 4 How to Design and Choose Cross-Linking Reagents
• 5 Homobifunctional Cross-Linking Reagents
• 6 Heterobifunctional Cross-Linkers
• 7 Multifunctional Cross-Linking Reagents
• 8 Monofunctional and Zero -Length Cross -Linking Reagents
• 9 General Approaches for Chemical Cross-Linking
• 10 Analysis of Cross-Linked Products
• 11 Applications of Chemical Cross-Linking to the Study of Biological Macromolecules
• 12 Applications of Chemical Conjugation in the Preparation of Immunoconjugates and Immunogens
• 13 Application of Chemical Conjugation for the Preparation of Immunotoxins and Other Drug Conjugates for Targeting Therapeutics
• 14 Application of Chemical Conjugation to Solid-State Chemistry
• Appendix A Amino Group-Directed Homobifunctional Cross-Linkers
• Appendix B Sulfhydryl Group-Directed Homobifunctional Cross-Linkers
• Appendix C Phenolate- and Imidazolyl-Group-Directed Reagents: Bisdiazonium Precursors
• Appendix D Group Selective Heterobifunctional Cross-Linkers
• Appendix E Photoactivatable Heterobifunctional Cross-Linking Reagents