Adenoviral Vectors for Gene Therapy

David T. Curiel, Alan L. Parker

• Publisher: Academic Press
• Year: 2025
• Edition: 3
• eBook ISBN:
  9780323958141
• Topic: Biological Sciences
• Subtopic: Genetics & Genomics
• Index: Biological Sciences

Adenoviral Vectors for Gene Therapy, Third Edition, provides detailed, comprehensive coverage of gene delivery vehicles based on the adenovirus that is emerging as an important tool in gene therapy. These exciting new therapeutic agents have great potential for the treatment of disease, as platforms for gene therapy and gene editing, as well as for oncology approaches, making them class leading agents in the gene-advanced therapies arena.The fully updated and expanded third edition covers the basic biology of adenoviruses and highlights the potential use of adenoviral vectors for the treatment of disease, including their construction, propagation, and purification, cutting-edge vectorology, and the use of adenoviral vectors in preclinical animal models. The book also considers the regulatory issues surrounding human clinical gene therapy trials. New chapters include adenoviral vaccines for veterinary applications, adenoviruses for gene editing, nonhuman primate adenoviruses, COVID-19 vaccines, vaccine applications, and oncolytic adenoviruses for antitumor immunization. This broad scope of information provides a solid overview of the field, allowing the reader to gain a complete understanding of the development and use of adenoviral vectors. - Provides complete coverage of the basic biology of adenoviruses, as well as their construction, propagation, and purification of adenoviral vectors - Introduces common strategies for the development of adenoviral vectors, along with cutting-edge methods for their improvement - Demonstrates noninvasive imaging of adenovirus-mediated gene transfer - Discusses the utility of adenoviral vectors in animal disease models - Considers Food and Drug Administration regulations for human clinical trials

Table of contents

• Adenoviral Vectors for Gene Therapy
• Chapter 1 Structural biology of adenovirus
• Chapter 2 Adenovirus cell entry
• Chapter 3 Adenovirus replication
• Chapter 4 Adenoviral vector construction I: Mammalian systems
• Chapter 5 Adenoviral vector construction II: Bacterial systems
• Chapter 6 Adenoviral vector packaging cell lines
• Chapter 7 Purification of adenovirus
• Chapter 8 Innate immune response to adenovirus vectors following intravascular administration
• Chapter 9 Antibodies against adenoviruses
• Chapter 10 Methods to mitigate immune response
• Chapter 11 Xenogenic adenoviral vectors as vaccines
• Chapter 12 Adenoviral vector targeting
• Chapter 13 Systems of adenoviral gene transfer vectors moving into their fourth generation
• Chapter 14 Hybrid adenoviral vectors
• Chapter 15 Canine adenovirus
• Chapter 16 Oncolytic adenovirus: Challenges and advancements of clinical translation
• Chapter 17 Imaging analysis of adenovirus particles during the cell entry and intracellular trafficking
• Chapter 18 Adenovirus-based strategies for cancer vaccines
• Chapter 19 Adenoviral vaccines—Infectious disease
• Chapter 20 Adenoviral vaccines: COVID-19
• Chapter 21 Adenovirus vectored veterinary vaccines
• Chapter 22 Utility of adenoviral vectors in animal models—CV disease
• Chapter 23 Utility of adenoviral vectors in animal models of human disease III: acquired diseases
• Chapter 24 Utility of adenoviral vectors in animal models of human disease: Genetic diseases
• Chapter 25 Adenoviral vectors for gene editing
• Chapter 26 Manufacturing and upscaling
• Chapter 27 Regulation of adenoviral vector-based therapies: A CBER/OTP perspective
• Index