Multimodal and Nanoscale Optical Microscopy
  1. 176 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

About this book

Optical microscopy is developing into nanoscopy and multimodal microscopy the better to decipher the functioning mechanisms in living systems, and investigating biological specimens at molecular level using fluorescence as a mechanism of contrast. Results have demonstrated the potential to provide information at the Angstrom level. Other optical methods now offer more in terms of spatial and temporal resolution, making it possible to study the delicate and complex relationship between structure and function in cells. Modern optical microscopes also use the decisive advantage provided by artificial intelligence algorithms. All in all, a rapidly changing field with an increasing number of questions to be answered. This book contains the lectures presented as Course 210 of the prestigious International School of Physics Enrico Fermi, Multimodal and Nanoscale Optical Microscopy, held in Varenna, Italy from 11 to 16 July 2021. Topics covered include fluorescence; linear and nonlinear microscopy; label-free with Mueller matrix and Brillouin microscopy; F-methods such as FRAP, FLIM, FRET and FCS; super-resolution, phototoxicity and photodamage; optical and magnetic trapping; image formation; and bioimage analysis, among others. The book also includes selected contributions of a number of young researchers. Packed with novel ideas, new instruments, challenging theoretical approaches and amazing applications, the book will be of interest to all those working in the field of multimodal and nanoscale optical microscopy.

Frequently asked questions

Yes, you can cancel anytime from the Subscription tab in your account settings on the Perlego website. Your subscription will stay active until the end of your current billing period. Learn how to cancel your subscription.
No, books cannot be downloaded as external files, such as PDFs, for use outside of Perlego. However, you can download books within the Perlego app for offline reading on mobile or tablet. Learn more here.
Perlego offers two plans: Essential and Complete
  • Essential is ideal for learners and professionals who enjoy exploring a wide range of subjects. Access the Essential Library with 800,000+ trusted titles and best-sellers across business, personal growth, and the humanities. Includes unlimited reading time and Standard Read Aloud voice.
  • Complete: Perfect for advanced learners and researchers needing full, unrestricted access. Unlock 1.4M+ books across hundreds of subjects, including academic and specialized titles. The Complete Plan also includes advanced features like Premium Read Aloud and Research Assistant.
Both plans are available with monthly, semester, or annual billing cycles.
We are an online textbook subscription service, where you can get access to an entire online library for less than the price of a single book per month. With over 1 million books across 1000+ topics, we’ve got you covered! Learn more here.
Look out for the read-aloud symbol on your next book to see if you can listen to it. The read-aloud tool reads text aloud for you, highlighting the text as it is being read. You can pause it, speed it up and slow it down. Learn more here.
Yes! You can use the Perlego app on both iOS or Android devices to read anytime, anywhere — even offline. Perfect for commutes or when you’re on the go.
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app.
Yes, you can access Multimodal and Nanoscale Optical Microscopy by P. Bianchini,A. Diaspro,C.J.R. Sheppard,Paolo Bianchini,Alberto Diaspro,Colin J.R. Sheppard,Margaux Bouzin in PDF and/or ePUB format, as well as other popular books in Physical Sciences & Physics. We have over one million books available in our catalogue for you to explore.

Information

Edition
1
Subtopic
Physics
Index
Physics

Table of contents

  1. Title Page
  2. Contents
  3. P. Bianchini, A. Diaspro, C.J.R. Sheppard and M. Bouzin - Preface
  4. Course group shot
  5. Colin J.R. Sheppard - Imaging in image scanning microscopy: A review
  6. Barbara Storti and Ranieri Bizzarri - Theory of photochromic Forster energy transfer
  7. Giuseppe Vicidomini - Single-photon laser-scanning microscopy
  8. Luca Lanzano, Elena Cerutti, Elisabetta Di Franco, Anna Provvidenza Privitera and Alberto Diaspro - Super-resolution imaging using fluorescence lifetimes and phasor analysis
  9. M. Mazzocca and D. Mazza - Exploring the eukaryotic nucleus by multimodal single molecule microscopy
  10. Laura Finzi and David Dunlap - The tethered particle motion and magnetic tweezer techniques: An introduction and some applications
  11. Antonio Fiore and Giuliano Scarcelli - Biophysical contrast mechanisms of Brillouin microscopy
  12. Laura Sironi, Mario Marini, Margaux Bouzin, Laura D?Alfonso, Maddalena Collini, Giuseppe Chirico, Claudio Conci, Emanuela Jacchetti, Manuela Teresa Raimondi, Alessandra Nardini, Giulio Cerullo, Rebeca Martinez Vazquez and Roberto Osellame - Laser microfabrication for optical sensing and imaging in vivo
  13. Peter Saggau - Dynamic diffractive optical elements in microscopy and nanoscopy
  14. F. Balzarotti - Information theory for single-molecule observation: MINFLUX localization
  15. Posters
  16. List of participants