Essential Laboratory Techniques and Biochemical Analysis

Darshan Malik, Nandita Narayanasamy, V.A. Pratyusha, V A Pratyusha

• Publisher: CRC Press
• Year: 2025
• eBook ISBN: 9781040392874
• Topic: Biological Sciences
• Subtopic: Microbiology
• Index: Biological Sciences

This laboratory manual comprehensively reviews essential laboratory practices and different biochemistry protocols. The initial chapters of the book provide an overview of lab safety protocols, focusing on the importance of accuracy and precision in experimental procedures. It covers essential topics, such as laboratory setup, proper handling and maintenance of lab apparatus, and waste disposal. It provides a detailed exploration of spectrophotometry principles and assays, along with comprehensive cell biology techniques, including staining and microscopy. The book also addresses qualitative and quantitative analyses of carbohydrates, amino acids, proteins, and lipids, providing methods for extraction and characterization. It further details the extraction, purification, and characterization of enzymes and presents enzymatic assays and studies on enzyme kinetics, providing a comprehensive understanding of enzyme activity and regulation. The final section introduces hematology techniques, including blood smear preparation and various blood parameter determinations. It also covers forensic tests for blood detection and serum protein electrophoresis. This book is useful for graduate and postgraduate students of biochemistry, molecular biology, and microbiology.

Table of contents

• Cover Page
• Half-Title Page
• Title Page
• Copyright Page
• Dedication Page
• Contents
• Preface
• Acknowledgement
• About the Editors
• Chapter 1 Laboratory Maintenance and Safety
• Chapter 2 Solutions, pH, and Buffers
• Chapter 3 UV-Vis Spectrophotometry and Spectrofluorimetry
• Chapter 4 Cell Biology
• Chapter 5 Carbohydrates
• Chapter 6 Amino Acids and Proteins
• Chapter 7 Lipids
• Chapter 8 Nucleic Acids
• Chapter 9 Enzymes
• Chapter 10 Hematology
• Appendix I: Common Buffers Used in the Laboratory
• Index