Bioanalytical Techniques

Principles and Applications

Tariq Altalhi, Abeer Alosaimi, Jorddy Neves Cruz

• Publisher: Wiley-Scrivener
• Year: 2025
• Print ISBN: 9781394314102
• eBook ISBN: 9781394314126
• Edition: 1
• Topic: Technology & Engineering
• Subtopic: Chemical & Biochemical Engineering
• Index: Technology & Engineering

The book provides a comprehensive guide that covers the fundamental principles and methodologies of essential bioanalytical techniques.

Bioanalytical Techniques: Principles and Applications is a comprehensive and authoritative book that explores the principles, methodologies, and applications of bioanalytical techniques in the field of life sciences. The book covers a wide range of analytical techniques used for the characterization, quantification, and analysis of biological samples, including proteins, nucleic acids, metabolites, and biomarkers. Using a multidisciplinary approach by integrating concepts from biochemistry, molecular biology, analytical chemistry, and biotechnology, this book provides a solid foundation in the fundamental principles underlying various bioanalytical techniques, such as spectroscopy, chromatography, electrophoresis, immunoassays, mass spectrometry, and biosensors. Each technique is explained in detail, including its working principles, instrumentation, data analysis, and practical considerations. The book incorporates case studies, examples, and practical tips to illustrate how these techniques are used to solve biological problems and address research questions. It also discusses emerging trends and technologies in bioanalytical techniques, such as microfluidics, nanotechnology, and omics approaches.

Readers will find the book:

• Offers comprehensive coverage of bioanalytical techniques, encompassing a wide range of methodologies, instruments, and applications through real-world case studies;
• Adopts a multidisciplinary approach, integrating concepts from biochemistry, molecular biology, analytical chemistry, and biotechnology;
• Explores emerging trends and technologies in bioanalytical techniques, such as microfluidics, nanotechnology, omics approaches, and bioinformatics;
• Includes practical guidance, troubleshooting tips, and common challenges in bioanalysis, equipping readers with valuable insights and strategies for successful experimentation and data interpretation;
• Features contributions from renowned experts and leaders in the field, ensuring the content is authoritative, up-to-date, and reflects the latest advancements in bioanalytical techniques.

Audience

Biochemists, biologists, chemists, and medical and pharmaceutical professionals interested in biomolecules, enzymology, and biochemical pathways.

Table of contents

• Cover
• Series Page
• Title Page
• Copyright Page
• Contents
• Preface
• Chapter 1 Point-of-Care Diagnostics for Rapid Disease Detection
• Chapter 2 Principles of Molecular Biology for Bioanalysis
• Chapter 3 UV–Visible Spectroscopy for Quantitative Analysis of Biomolecules
• Chapter 4 Emerging Trends and Future Perspectives in Bioanalysis
• Chapter 5 Introduction to Biosensors for Point-of-Care Testing: IoT-Based Vitamin D3 Biosensor
• Chapter 6 Fluorescence Spectroscopy for Protein–Protein Interactions
• Chapter 7 Flow Cytometry for Cell Analysis and Sorting
• Chapter 8 Application in Drug Discovery and Development for Lead Identification
• Chapter 9 Immunoblotting Techniques for Protein Identification and Quantification and Its Application
• Chapter 10 Gel Electrophoresis for DNA Fragment Analysis
• Chapter 11 Omics Approaches in Bioanalysis for Systems Biology Studies
• Chapter 12 Introduction to Mass Spectrometry for Metabolite Profiling
• Chapter 13 Infections, Symptoms, and Clinical Diagnostic Techniques for Dengue: A Case Study of a Neglected Tropical Disease
• Chapter 14 Microfluidic-Based Bioassays for High-Throughput Analysis
• Chapter 15 Circular Dichroism Spectroscopy for Secondary Structure Analysis of Proteins
• Chapter 16 Optical Biosensors for Detection of Analytes in Biological Samples
• Chapter 17 Tandem Mass Spectrometry for Peptide Sequencing
• Chapter 18 Unraveling Complexity: Cutting-Edge Ion Chromatography Methods for the Analysis of Inorganic Ions in Biological Samples
• Chapter 19 Sample Preparation and Handling for Reliable Results
• Chapter 20 Data Analysis in Bioanalysis for Interpretation of Experimental Data
• Chapter 21 Protein Identification Using Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry
• Chapter 22 Applications of Electrothermal Atomic Absorption Spectrometry (ET-AAS) Toward Various Metal Analyses in Diverse Biological and Environmental Matrices
• Chapter 23 Thin-Layer Chromatography for Natural Product Analysis
• Index
• EULA