Protocols in Root-Microbe Interactions describes laboratory protocols that have been developed and optimized for scientists working in root-microbe interactions, specifically for those wanting to quantify immune responses in plant roots. The experiments are focused on cellular to whole-root scale and include molecular biology, biochemistry, and histology techniques. Written in an easy-to-understand fashion that is suitable for both early career and experienced scientists, the protocols were optimized for tomato roots but can easily be adapted to roots of other plant species, or if needed, to leaves. The book includes information on quantifying pattern-triggered immune (PTI) responses in plant roots and covers reactive oxygen species (ROS) burst, mitogen-activated protein kinase (MAPK) phosphorylation, root growth inhibition, and qualitative callose deposition. Other topics examined include transient expression of bacterial proteins in leaves and root sectioning (from a microtome and vibratome) and histology assays for both light microscopy and scanning electron microscopy. A variety of other protocols explored relate to the isolation of bacteria from the root endosphere and rhizosphere, and the extraction of DNA from root-associated bacteria. Protocols in Root-Microbe Interactions serves as an invaluable resource for those working in the root-microbe field and can be optimized for other laboratories' needs.
- 131 pages
- English
- PDF
- Available on iOS & Android
eBook - PDF
Protocols in Root-Microbe Interactions
About this book
Trusted by 375,005 students
Access to over 1 million titles for a fair monthly price.
Study more efficiently using our study tools.
Information
Table of contents
- Cover
- PROTOCOLS IN ROOT-MICROBE INTERACTIONS
- Title
- Copyright
- CONTENTS
- Preface and Acknowledgments
- Introduction
- PART 1. PROTOCOLS FOR PATTERN-TRIGGERED IMMUNITY ASSAYS IN ROOTS
- PART 2. HISTOLOGY
- PART 3. FUNCTIONAL ASSAYS FOR ROOT-ASSOCIATED BACTERIA
- PART 4. RNA EXTRACTION PROTOCOLS FOR ROOTS
- PART 5. COMMONLY USED AGAR RECIPES FOR TOMATO AND BACTERIAL GROWTH
- About the Author
- About the Contributors
Frequently asked questions
Yes, you can cancel anytime from the Subscription tab in your account settings on the Perlego website. Your subscription will stay active until the end of your current billing period. Learn how to cancel your subscription
No, books cannot be downloaded as external files, such as PDFs, for use outside of Perlego. However, you can download books within the Perlego app for offline reading on mobile or tablet. Learn how to download books offline
Perlego offers two plans: Essential and Complete
- Essential is ideal for learners and professionals who enjoy exploring a wide range of subjects. Access the Essential Library with 800,000+ trusted titles and best-sellers across business, personal growth, and the humanities. Includes unlimited reading time and Standard Read Aloud voice.
- Complete: Perfect for advanced learners and researchers needing full, unrestricted access. Unlock 1.4M+ books across hundreds of subjects, including academic and specialized titles. The Complete Plan also includes advanced features like Premium Read Aloud and Research Assistant.
We are an online textbook subscription service, where you can get access to an entire online library for less than the price of a single book per month. With over 1 million books across 990+ topics, we’ve got you covered! Learn about our mission
Look out for the read-aloud symbol on your next book to see if you can listen to it. The read-aloud tool reads text aloud for you, highlighting the text as it is being read. You can pause it, speed it up and slow it down. Learn more about Read Aloud
Yes! You can use the Perlego app on both iOS and Android devices to read anytime, anywhere — even offline. Perfect for commutes or when you’re on the go.
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app
Yes, you can access Protocols in Root-Microbe Interactions by Anjali S. Iyer-Pascuzzi in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Cell Biology. We have over one million books available in our catalogue for you to explore.