Photonic Imaging for Biology
eBook - ePub

Photonic Imaging for Biology

From Conventional Microscopy to Super-Resolution

  1. English
  2. ePUB (mobile friendly)
  3. Available on iOS & Android
eBook - ePub

Photonic Imaging for Biology

From Conventional Microscopy to Super-Resolution

About this book

Light microscopy is a central tool in biological research, allowing scientists to observe living cells and organisms with details invisible to the naked eye. Since its inception in the 17th century, it has evolved through key innovations in optics, staining, electronics and informatics. Major milestones include phase contrast, differential interference contrast, immunofluorescence, genetically encoded fluorescent proteins, confocal microscopy and super-resolution microscopy.

The discovery of Green Fluorescent Protein (GFP) revolutionized molecular biology, while 21st-century advances, such as super-resolution microscopy and artificial intelligence, have pushed imaging capabilities even further. Modern microscopes now integrate digital imaging, advanced optics and computational analysis for enhanced visualization and interpretation.

Photonic Imaging for Biology outlines major microscopy techniques that have driven biological discoveries, starting with fundamental principles and covering a range of methods, including brightfield, fluorescence, confocal, light-sheet, single particle tracking, photoperturbation, fluorescence correlation spectroscopy and super-resolution microscopy. The book concludes with a chapter on image analysis, highlighting recent progress in artificial intelligence. Each chapter focuses on specific techniques and their applications, strengths and limitations.

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Yes, you can access Photonic Imaging for Biology by Jean-Baptiste Sibarita in PDF and/or ePUB format, as well as other popular books in Physical Sciences & Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Wiley-ISTE
Year
2025
Print ISBN
9781789452228
eBook ISBN
9781394417889
Edition
1
Subtopic
Biology

Table of contents

  1. Cover
  2. Table of Contents
  3. Title Page
  4. Copyright Page
  5. Preface Photonic Microscopy for Biology: From Microscopy to Super-Resolution
  6. 1 Principles of Light Microscopy
  7. 2 Contrast-based Label-Free Imaging and Phase Measurement
  8. 3 Fluorophores and Labeling Methods for Fluorescence Microscopy
  9. 4 Quantitative FRAP and FCS
  10. 5 Single-Particle Tracking for Nanoscale Dynamics of Biological Samples
  11. 6 In Depth Microscopy
  12. 7 Structured Illumination Microscopy
  13. 8 STED Imaging of Neuronal Morphology
  14. 9 Single-Molecule Localization Microscopy: From Imaging Cellular Structures to Quantitative Image Analysis
  15. 10 Image Processing and Image Analysis in Microscopy
  16. List of Authors
  17. Index
  18. End User License Agreement