Techniques and Protocols in Molecular Biology
eBook - ePub

Techniques and Protocols in Molecular Biology

  1. English
  2. ePUB (mobile friendly)
  3. Available on iOS & Android
eBook - ePub

Techniques and Protocols in Molecular Biology

About this book

Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work. - Provides step-by-step instructions to guide researchers in carrying out a range of molecular biology techniques - Includes details of instrumentation, reagents, and buffers necessary for each experiment - Features diagrams to help illustrate the procedures and techniques covered

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Information

Publisher
Academic Press
Year
2025
eBook ISBN
9780443141614
Topic
Biowissenschaften
Subtopic
Molekularbiologie

Table of contents

  1. Cover image
  2. Title page
  3. Copyright
  4. Table of Contents
  5. Biography
  6. CHAPTER 1 Genomic DNA isolation (Bacteria, plant, and animal cells/tissues)
  7. Chapter 2 RNA isolation from mammalian cells/tissues, Drosophila, and Caenorhabditis elegans using monophasic lysis reagent
  8. CHAPTER 3 Reverse-transcription polymerase chain reaction
  9. CHAPTER 4 Quantitative reverse-transcription polymerase chain reaction: Real-time polymerase chain reaction
  10. CHAPTER 5 Agarose gel electrophoresis
  11. CHAPTER 6 3′RACE (random amplification of cDNA end)
  12. CHAPTER 7 5′RACE (Random amplification of cDNA end)
  13. CHAPTER 8 Plasmid isolation and purification (alkaline lysis method)
  14. CHAPTER 9 Preparation of competent cells (Bacterial cells) and transformation (Hanahan's method)
  15. CHAPTER 10 Molecular gene cloning
  16. CHAPTER 11 End-labeling of DNA (5′end labeling by T4 polynucleotide kinase)
  17. CHAPTER 12 Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis
  18. CHAPTER 13 Western blotting
  19. CHAPTER 14 Electrophoretic mobility shift assay for DNA-protein interaction
  20. CHAPTER 15 Silver staining of proteins in acrylamide gel compatible with mass spectrometry: Acidic method
  21. CHAPTER 16 Animal cell culture (Adherent cells)
  22. CHAPTER 17 Cell viability by MTT assay
  23. CHAPTER 18 Lactate dehydrogenase assay
  24. CHAPTER 19 Luciferase assay
  25. CHAPTER 20 Co-immunoprecipitation
  26. CHAPTER 21 Protein purification (His-tagged proteins by immobilized metal affinity chromatography)
  27. CHAPTER 22 Subcellular fractionation by differential centrifugation (Separating nuclear, membrane, and cytosolic fraction)
  28. CHAPTER 23 DNase 1 footprinting
  29. CHAPTER 24 Chromatin immunoprecipitation-qPCR
  30. CHAPTER 25 Flow cytometry for cell surface markers
  31. CHAPTER 26 Basics of bioinformatics
  32. CHAPTER 27 GST pull-down assay
  33. CHAPTER 29 An overview of yeast two-hybrid assay
  34. CHAPTER 30 Enzyme-linked immunosorbent assay
  35. References
  36. Index

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Yes, you can access Techniques and Protocols in Molecular Biology by Buddhi Prakash Jain in PDF and/or ePUB format, as well as other popular books in Biowissenschaften & Molekularbiologie. We have over 1.5 million books available in our catalogue for you to explore.