Methods for the Study of Microbial Biofilms

Current Trends and Recent Advancements

Abhishek Guldhe, Vinothkannan Ravichandran, Shashank S Kamble

• Publisher: Academic Press
• Year: 2025
• Topic: Biological Sciences
• eBook ISBN:
  9780443218507
• Subtopic: Molecular Biology
• Index: Biological Sciences

Methods for the study of microbial biofilms: Current Trends and Recent Advancements provides a detailed overview of the latest techniques for the study of biofilms. The book explores a wide range of methods for the characterization and study of cells within biofilms, including microscopy techniques, genomic approaches, transcriptomics, proteomic and proteogenomic tools. Computational and mathematical modelling are also covered.Chapters investigate the physiology of biofilm cells, gene expression pathways related to the formation, maturation and dispersion of biofilms, and molecular factors governing the biofilm formation and development, providing an overview of techniques that can be used at the cellular and molecular level. This book provides a broad overview of the biofilm field, along with the range of techniques that can be used to study and better understand them. - Explores in silico, in vitro, and in vivo analytical techniques for investigating biofilms - Evaluates recent trends in omics approaches for biofilm characterization - Facilitates understanding of the 3D organization of cells within biofilms, cell to cell communication, and the microbial dynamics within the biofilm community - Looks at microbial biofilms across a range of disciplines, including those associated with medical devices, the food industry, and wastewater treatment

Table of contents

• Title of Book
• 1 Microbial biofilms: occurrence and implications
• 2 In silico methods to study biofilms
• 3 Microscopic methods for biofilm analysis
• 4 In vitro assays to study biofilms
• 5 In vivo animal models in biofilm research
• 6 Genomic approaches for biofilm analysis and control
• 7 Transcriptomics approaches to study biofilm: quantitative polymerase chain reaction-based methods
• 8 Decoding biofilm complexity through transcriptomics: microarray and RNA sequencing approaches
• 9 Proteomics studies of biofilm: methods, therapeutics, and recommendations
• 10 Metabolomics studies in microbial biofilms
• 11 Metagenomics/population studies for biofilms
• 12 Proteogenomics approaches toward biofilm formation studies
• 13 Mathematical tools to study and understand the biofilms
• Index