"Microbial Ecology: Culture-dependent Methods" is a comprehensive guide to studying microbial diversity. The book details methods for sampling and characterizing microbes from soil and water, covering both physicochemical and microbiological aspects. It delves into the cultivation, isolation, and preservation of bacteria and archaea, including cryopreservation and freeze-drying. Subsequent chapters focus on molecular techniques like DNA extraction, PCR, 16S rRNA gene sequencing, and bioinformatic analysis, along with advanced methods such as MLST, qPCR, and Whole Genome Sequencing. The book also covers sequence deposition and provides protocols for DGGE, making it an invaluable resource for microbial ecologists.
- 300 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
eBook - ePub
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Table of contents
- Cover Page
- Title Page
- Copyright Page
- Preface
- Contents
- Chapter 1 Samples and Sampling Methods for the Study of Microbial Diversity from Soil and Water
- Chapter 2 Physicochemical Characterization of Soil and Water Samples
- Chapter 3 Microbiological Characterization of Soil and Water Samples
- Chapter 4 Cultivation, Isolation, and Pure Culture of Bacteria and Archaea
- Chapter 5 Short-term and Long-term Preservation of Microorganisms at Extremely Low Temperatures
- Chapter 6 Long-term Preservation of Microorganisms using Freeze-drying
- Chapter 7 Biochemical Characterization of Bacteria
- Chapter 8 Tests for Screening Aminopeptidase, KOH Test, and Antimicrobial Resistance
- Chapter 9 Bacterial DNA Extraction
- Chapter 10 A Detailed Method of Bacterial DNA Extraction using Sonication
- Chapter 11 DNA Quantification and Agarose Gel Electrophoresis
- Chapter 12 Amplification of the 16S rRNA using Polymerase Chain Reaction (PCR)
- Chapter 13 PEG-NaCl Purification of PCR Products: Principles, Protocols, and Applications
- Chapter 14 16S rRNA Gene Sequencing Method and Bioinformatic Analysis
- Chapter 15 Software Tools for Sequence Analysis and Identification
- Chapter 16 Deposition of Sequence Data in INSDC, ENA, and DDBJ Repositories
- Chapter 17 Deposition of Pure Cultures in International Culture Collections
- Chapter 18 Primer Design for Polymerase Chain Reactions (PCRs) and Sequencing PCRs
- Chapter 19 Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Microbial Gene Detection
- Chapter 20 Multilocus Sequence Typing (MLST) for Microbial Identification and Classification
- Chapter 21 Quantitative Polymerase Chain Reaction (qPCR) Protocol and Applications
- Chapter 22 Whole Genome Sequencing (WGS) of Bacteria
- Chapter 23 Bacterial and Archaeal Genome Annotation
- Chapter 24 Denaturing Gradient Gel Electrophoresis (DGGE) Protocol and its Applications in Microbial Ecology
- Index
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Yes, you can access Microbial Ecology by Bhagwan Narayan Rekadwad in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biotechnology. We have over 1.5 million books available in our catalogue for you to explore.