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Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures
Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures

Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures

Paul Monis, Nic Reid
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33 pages
language
English
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PDF
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Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures

Paul Monis, Nic Reid
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About This Book

Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP's developed.
This book is co-published with Water Research Australia.

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Information

Publisher
IWA Publishing
Year
2015
ISBN
9781780407524
Topic
Technology & Engineering
Subtopic
Applied Sciences
Edition
1

Table of contents