The lac Operon
eBook - PDF

The lac Operon

  1. 216 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

The lac Operon

About this book

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Yes, you can access The lac Operon by Benno Müller-Hill in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biochemistry. We have over one million books available in our catalogue for you to explore.

Information

Publisher
De Gruyter
Year
2011
Print ISBN
9783110148305
eBook ISBN
9783110879476
Edition
1
Topic
Biological Sciences
Subtopic
Biochemistry
Index
Biological Sciences

Table of contents

  1. Introduction
  2. Part 1: A Short History of the lac System from its Beginning to 1978
  3. 1.1 From Noah to Pasteur: Adaptation in Yeast
  4. 1.2 Adaptation in Bacillus subtilis and Escherichia coli
  5. 1.3 Mutants in the lac System of Escherichia coli
  6. 1.4 Inducers of the lac System and the Discovery of Permease
  7. 1.5 Negative Control through Repressor
  8. 1.6 Further Evidence for Negative Control through Repressor
  9. 1.7 The Triumph of the Jacob-Monod Theory
  10. 1.8 Minor Defects in the Jacob-Monod Theory
  11. 1.9 Isolation of the Lac and λ CI Repressors
  12. 1.10 Putting the lac Genes on Phage φ80 or λ
  13. 1.11 Lac and λ CI Repressors Bind to their Operator DNAs
  14. 1.12 Making Grams of Lac Repressor to Determine its Sequence
  15. 1.13 Mutations in lacI Suggest a Modular Structure of Repressor
  16. 1.14 Miller’s Analysis of the I Gene: Climbing Mount Everest
  17. 1.15 Positive Control through the CAP Protein
  18. 1.16 Isolation and Sequence of lac Operator
  19. 1.17 Chemical DNA Sequencing
  20. 1.18 Steric Hindrance is the Mechanism of Repression
  21. 1.19 Chemical Synthesis of lac Operator DNA
  22. 1.20 Lac Repressor-Operator Complex Binds to Nitrocellulose
  23. 1.21 Polyacrylamide Gel Electrophoresis of Protein-DNA Complexes
  24. 1.22 Fusion of and Complementation within the lacZ Gene
  25. 1.23 Isolation of Lac Permease
  26. 1.24 The End of an Era
  27. Part 2: Misinterpretation
  28. 2.1 The Interpretation of Experiments is Sometimes Wrong
  29. 2.2 Adaptation Explained by Self-Replicating Genes
  30. 2.3 The Kinetics of Adaptation
  31. 2.4 Lac Repressor is RNA
  32. 2.5 Operator is RNA or Protein
  33. 2.6 Isolation of Lac Repressor
  34. 2.7 Isolation of Lac Permease and Arg Repressor
  35. 2.8 Genetic Proof that lac Operator is Dyadic
  36. 2.9 The Core of Lac Repressor Binds to lac Operator
  37. 2.10 Lac Repressor Uses ß-Sheet to Recognize lac Operator
  38. 2.11 A π-Helix Binds to a Hairpin Loop of lac Operator
  39. 2.12 Prediction of the Sequence of lac Operator
  40. 2.13 The Kinetics of lac Repressor-Operator Interaction
  41. 2.14 Lac Repressor Bound to O2 Acts as an Efficient Road Block
  42. 2.15 Auxiliary Operators Can be Disregarded in Repression
  43. 2.16 CAP Activates Transcription like λ CI Repressor
  44. 2.17 In the Pitfall of Symmetry
  45. 2.18 The CAP-DNA Complex: Two Propositions
  46. 2.19 The Adenine of Cyclic AMP Binds to DNA
  47. 2.20 The Structure of ß-Galactosidase
  48. 2.21 From Lamarck to Cairns
  49. 2.22 DNA Sequence Analysis: Not in this Century!
  50. 2.23 Protein Splicing
  51. 2.24 OI-Repressor-O2 Loops
  52. Part 3: The lac Operon, a Paradigm of Beauty and Efficiency
  53. 3.1 The New Perspective
  54. 3.2 Some Numbers and Concepts
  55. 3.3 Activation of the lac Promoter by CAP Protein
  56. 3.4 Repression of the lac Promoter by Lac Repressor
  57. 3.5 ß-Galactosidase, Lac Permease and Transacetylase
  58. 3.6 The lac System as a Tool
  59. 3.7 What Lesson can be Learned from the lac System?
  60. 3.8 Outlook
  61. Authors Index
  62. Subject Index