Fetal Cells and Fetal DNA in Maternal Blood
New Developments for a New Millennium. 11th Fetal Cell Workshop, Basel, April 2000: Proceedings
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Fetal Cells and Fetal DNA in Maternal Blood
New Developments for a New Millennium. 11th Fetal Cell Workshop, Basel, April 2000: Proceedings
About this book
Is noninvasive, risk-free prenatal diagnosis of fetal genetic characteristics still a fantasy, or will it soon become reality? The current status of both the leading European research groups as well as the NIH-funded NIFTY study are reported here, indicating that certain fetal genetic traits can now be examined efficiently in a noninvasive manner. Considerable focus is placed on new laser-mediated systems for the effective micro-manipulation of single fetal cells, as well as their analysis by single-cell PCR and the pitfalls to avoid when performing such analyses. Other issues addressed in depth include: novel enrichment techniques, optimal fetal cell recognition, fetal cell culture, as well as the exciting finding that fetal cell traffic is elevated in certain pregnancy-related disorders, most prominently in preeclampsia. This publication is of interest to researchers in the field, genetic counsellors, gynecologists and obstetricians, and researchers in microchimerism, transplantation and transfusion medicine.
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Information
Table of contents
- Cover
- Contents
- Preface
- Circulating DNA in Plasma or Serum
- Experience with Single Cell PCR in Preimplantation Genetic Diagnosis: How to Avoid Pitfalls
- Laser-Mediated Micromanipulation Systems: An Important Tool for the Analysis of Single Cells
- Why Do We Need Noninvasive Prenatal Diagnostic Procedures?
- Fetal Cells in Maternal Blood: An Overview of the Basel Experience
- Fetal Cells in Maternal Blood at Sheba Medical Center, Israel: Analysis of Three Cell Types during the Three Trimesters of Pregnancy
- Nucleated Red Blood Cell Recovery from Maternal Blood with Non-Commercial Monoclonal Antibodies
- How to Enrich and Analyze Fetal Cells from Maternal Blood: 5- Year Experience with PCR, FISH and Cultivation
- Solid Phase Method for Enrichment of Fetal Cells from Maternal Blood
- Fetal Nucleated Red Blood Cells in Maternal Blood during Pregnancy: First Czech Experience
- Using AMCA to Label the Fetal Cell Antigen in Fetal Erythroblasts Circumvents Heme Autofluorescence, Enhances cFISH Efficienc
- Fetal Cell Detection in Maternal Blood Using the Solid Phase Cytometer Chem Scan ® RDI: Preliminary Studies
- Fetal CD34+ Cell Culture and Identification from Maternal Blood
- Free Fetal DNA in Maternal Plasma and Serum: Diagnostic and Clinical Implications
- Quantitative Analysis of Fetal DNA in Maternal Plasma in Preeclamptic Pregnancies
- Circulating Trophoblast Cells in Maternal Peripheral Blood
- Transcervical Sampling
- Impact of Improved Screening Efficiency on the Future Role of Non-Invasive Testing
- Author Index
- Subject Index