Ultrastructure of Mast Cells and Basophils

A. M. Dvorak, Renz, T. A. E. Platts-Mills

• Publisher: S. Karger
• Year: 2005
• Print ISBN:
  9783805578646
• eBook ISBN:
  9783318011753
• Edition: 1
• Topic: Medizin
• Subtopic: Dermatologie

Basophils and mast cells are similar but unique secretory cells which play a central role in inflammatory and immediate allergic reactions. In this book, the author reviews the ultrastructural studies of basophils and mast cells performed since the publication of an earlier monograph in 1991. These studies include the use of ultrastructural rules that allow identification of basophils and mast cells in new circumstances. The secretory granules and lipid bodies present in these cells are established as different, important organelles. A number of standard and newly developed ultrastructural imaging methods are described which define the subcellular locations of chymase, Charcot-Leyden crystal protein, histamine, and heparin as well as materials important in arachidonate and RNA metabolism, and in cytokine biology in basophils and mast cells. The findings presented elucidate new aspects of the secretory mechanisms of basophils and mast cells, their recovery, and the transepithelial route of passage of macromolecules stimulated by permeability mediators released from these cells. Including more than 180 excellent micrographs, this book will be of special interest to basic scientists in immunology, cell biology, secretion and angiogenesis research, and histochemistry. As mast cells and basophils play a crucial role in various diseases, it will be essential reading for allergists, immunologists, dermatologists, hematologists, gastroenterologists, rheumatologists, neurologists, pathologists, pulmonologists, and cardiologists.

Table of contents

• Cover
• Contents
• Preface
• Abbreviations
• Introduction
• Ultrastructural Analysis Is Necessary and Suffi cient for Identifi cation of Basophils and Mast Cells
• Cyclooxygenase, a Key Enzyme Family for Production of Prostaglandins, Is Present in Human Mast Cell Lipid Bodies
• Subcellular Localization of the Cytokines, Basic Fibroblast Growth Factor and Tumor Necrosis Factor- in Mast Cells
• Immunogold Ultrastructural Techniques Identify Subcellular Sites of Chymase, Charcot-Leyden Crystal Protein, and Histamine in Basophils and Mast Cells
• Ultrastructural Enzyme-Affi nity-Gold and Inhibitor-Gold Techniques Identify Subcellular Sites of Histamine and Heparin in Basophils and Mast Cells
• Piecemeal Degranulation of Basophils and Mast Cells Is Effected by Vesicular Transport of Stored Secretory Granule Contents
• Mast Cell-Derived Mediators of Enhanced Microvascular Permeability, Vascular Permeability Factor/ Vascular Endothelial Growth Factor, Histamine, and Serotonin, Cause Leakage of Macromolecules through a New Endothelial Cell Permeability Organelle, the Vesiculo-Vacuolar Organelle
• Degranulation and Recovery from Degranulation of Basophils and Mast Cells
• Mast Cell Secretory Granules and Lipid Bodies Contain the Necessary Machinery Important for the in situ Synthesis of Proteins
• Concluding Statement
• References
• Subject Index